Zwitterionic betaine transition from micelles to vesicles induced by cholesterol

R. Alenaizi, Shahidan Radiman, Irman Abdul Rahman, Faizal K.P. Kunchi Mohamed

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Vesicle formation by self-assembly in an aqueous mixture of zwitterionic carboxylate betaine surfactant and cholesterol was studied as a potential drug delivery nanocarrier. In this study, pyrene and 1,6-Diphenyle1-1,3,5-hexatrine (DPH) were applied as fluorescence probes that provides information on polarity and fluidity of the microenvironment, and used to monitor the transition of zwitterionic betaine from monomers to micelles, and to vesicles promoted by addition of cholesterol to micellar solutions through the variation of vibronic peak intensity ratio I1/I3 of the pyrene and solubilization with intensity quenching I428 of (DPH). In addition, the steady-state and time-resolved fluorescence anisotropy measurements of the (DPH) probe provides information on the effect of cholesterol on the dynamic properties vesicle membrane and vesicular distribution accompanied by dynamic light scattering (DLS), transmission electron microscopy (TEM), and turbidity measurements. Our results show an abrupt change of the ratio I1/I3 of pyrene and fluorescence intensity I428 of (DPH) in the transition from monomer to micelle with increasing betaine concentration, as well as fluorescence anisotropy results thought the transition from micelle to vesicle promoted by cholesterol and confirmed by TEM result. Furthermore, the fluorescence anisotropy measurements shows that addition of small amount of cholesterol to zwitterionic betaine micelle have a significant influence on the molecular packing leading to vesicle formation and increasing bilayer rigidity with increasing cholesterol concentration up to χchol = 0.25.

Original languageEnglish
Pages (from-to)1226-1233
Number of pages8
JournalJournal of Molecular Liquids
Volume223
DOIs
Publication statusPublished - 1 Nov 2016

Fingerprint

Betaines
Betaine
betaines
Cholesterol
cholesterol
Micelles
micelles
Fluorescence
Pyrene
pyrenes
fluorescence
Anisotropy
anisotropy
monomers
Monomers
Transmission electron microscopy
transmission electron microscopy
Fluidity
probes
turbidity

Keywords

  • Anisotropy
  • Betaine
  • Cholesterol
  • DPH
  • Pyrene
  • Vesicle
  • Zwitterionic surfactant

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

Zwitterionic betaine transition from micelles to vesicles induced by cholesterol. / Alenaizi, R.; Radiman, Shahidan; Abdul Rahman, Irman; K.P. Kunchi Mohamed, Faizal.

In: Journal of Molecular Liquids, Vol. 223, 01.11.2016, p. 1226-1233.

Research output: Contribution to journalArticle

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