Xylooligosaccharides from potential agricultural waste: Characterization and screening on the enzymatic hydrolysis factors

Nurul Aishah Mazlan, Kamaliah Abdul Samad, Hafizuddin Wan Yussof, Syed Mohd Saufi, Jamaliah Md Jahim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The interest of lignocellulosic biomass to be utilized in bioconversion sectors has gaining much attention recently. As corresponding to this, an experimental study was done to investigate the potential agricultural waste for xylan preparation and to screen the factors affecting xylooligosaccharides (XOS) production. Characterization of oil palm frond bagasse (OPFB), oil palm empty fruit bunch (OPEFB), and rice straw (RS) before and after pretreatment was performed in terms of chemical and physical properties. Enzymatic hydrolysis using a 25−1 fractional factorial design was further conducted to evaluate the effect of solid loading, enzyme loading, temperature, hydrolysis time and agitation on XOS production from the selected agricultural waste. From characterization results OPFB was determined as the greatest promising agricultural waste for XOS production. Pretreatment on OPFB simultaneously exhibited the highest increase of xylan recovery and lignin removal. XOS production up to 174.74 mg/g through enzymatic hydrolysis was successfully achieved from the pretreated OPFB. The enzymatic hydrolysis conditions of 1% solid loading and 50 U/mL enzyme loading at 55 °C temperature for 4 h hydrolysis time with 50 rpm of agitation were found efficient to obtain maximum XOS production. Among those factors solid loading was determined as the most significant factor that contributed to the increase of XOS production. The findings from this study have verified that acid pretreatment could improve the biomass structure by disrupting its structure which is important to facilitate enzymatic hydrolysis for high yield of XOS production.

Original languageEnglish
Pages (from-to)575-584
Number of pages10
JournalIndustrial Crops and Products
Volume129
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

xylooligosaccharides
agricultural wastes
enzymatic hydrolysis
Elaeis guineensis
bagasse
screening
fronds
pretreatment
xylan
agitation
hydrolysis
biomass
rice straw
enzymes
biotransformation
lignin
physical properties
temperature
physicochemical properties
fruits

Keywords

  • Acid pretreatment
  • Enzymatic hydrolysis
  • Oil palm frond bagasse
  • Xylan recovery
  • Xylooligosaccharides

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Xylooligosaccharides from potential agricultural waste : Characterization and screening on the enzymatic hydrolysis factors. / Mazlan, Nurul Aishah; Samad, Kamaliah Abdul; Wan Yussof, Hafizuddin; Saufi, Syed Mohd; Md Jahim, Jamaliah.

In: Industrial Crops and Products, Vol. 129, 01.03.2019, p. 575-584.

Research output: Contribution to journalArticle

Mazlan, Nurul Aishah ; Samad, Kamaliah Abdul ; Wan Yussof, Hafizuddin ; Saufi, Syed Mohd ; Md Jahim, Jamaliah. / Xylooligosaccharides from potential agricultural waste : Characterization and screening on the enzymatic hydrolysis factors. In: Industrial Crops and Products. 2019 ; Vol. 129. pp. 575-584.
@article{ea37e35382bb42cd814e328b9d991261,
title = "Xylooligosaccharides from potential agricultural waste: Characterization and screening on the enzymatic hydrolysis factors",
abstract = "The interest of lignocellulosic biomass to be utilized in bioconversion sectors has gaining much attention recently. As corresponding to this, an experimental study was done to investigate the potential agricultural waste for xylan preparation and to screen the factors affecting xylooligosaccharides (XOS) production. Characterization of oil palm frond bagasse (OPFB), oil palm empty fruit bunch (OPEFB), and rice straw (RS) before and after pretreatment was performed in terms of chemical and physical properties. Enzymatic hydrolysis using a 25−1 fractional factorial design was further conducted to evaluate the effect of solid loading, enzyme loading, temperature, hydrolysis time and agitation on XOS production from the selected agricultural waste. From characterization results OPFB was determined as the greatest promising agricultural waste for XOS production. Pretreatment on OPFB simultaneously exhibited the highest increase of xylan recovery and lignin removal. XOS production up to 174.74 mg/g through enzymatic hydrolysis was successfully achieved from the pretreated OPFB. The enzymatic hydrolysis conditions of 1{\%} solid loading and 50 U/mL enzyme loading at 55 °C temperature for 4 h hydrolysis time with 50 rpm of agitation were found efficient to obtain maximum XOS production. Among those factors solid loading was determined as the most significant factor that contributed to the increase of XOS production. The findings from this study have verified that acid pretreatment could improve the biomass structure by disrupting its structure which is important to facilitate enzymatic hydrolysis for high yield of XOS production.",
keywords = "Acid pretreatment, Enzymatic hydrolysis, Oil palm frond bagasse, Xylan recovery, Xylooligosaccharides",
author = "Mazlan, {Nurul Aishah} and Samad, {Kamaliah Abdul} and {Wan Yussof}, Hafizuddin and Saufi, {Syed Mohd} and {Md Jahim}, Jamaliah",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.indcrop.2018.12.042",
language = "English",
volume = "129",
pages = "575--584",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

TY - JOUR

T1 - Xylooligosaccharides from potential agricultural waste

T2 - Characterization and screening on the enzymatic hydrolysis factors

AU - Mazlan, Nurul Aishah

AU - Samad, Kamaliah Abdul

AU - Wan Yussof, Hafizuddin

AU - Saufi, Syed Mohd

AU - Md Jahim, Jamaliah

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The interest of lignocellulosic biomass to be utilized in bioconversion sectors has gaining much attention recently. As corresponding to this, an experimental study was done to investigate the potential agricultural waste for xylan preparation and to screen the factors affecting xylooligosaccharides (XOS) production. Characterization of oil palm frond bagasse (OPFB), oil palm empty fruit bunch (OPEFB), and rice straw (RS) before and after pretreatment was performed in terms of chemical and physical properties. Enzymatic hydrolysis using a 25−1 fractional factorial design was further conducted to evaluate the effect of solid loading, enzyme loading, temperature, hydrolysis time and agitation on XOS production from the selected agricultural waste. From characterization results OPFB was determined as the greatest promising agricultural waste for XOS production. Pretreatment on OPFB simultaneously exhibited the highest increase of xylan recovery and lignin removal. XOS production up to 174.74 mg/g through enzymatic hydrolysis was successfully achieved from the pretreated OPFB. The enzymatic hydrolysis conditions of 1% solid loading and 50 U/mL enzyme loading at 55 °C temperature for 4 h hydrolysis time with 50 rpm of agitation were found efficient to obtain maximum XOS production. Among those factors solid loading was determined as the most significant factor that contributed to the increase of XOS production. The findings from this study have verified that acid pretreatment could improve the biomass structure by disrupting its structure which is important to facilitate enzymatic hydrolysis for high yield of XOS production.

AB - The interest of lignocellulosic biomass to be utilized in bioconversion sectors has gaining much attention recently. As corresponding to this, an experimental study was done to investigate the potential agricultural waste for xylan preparation and to screen the factors affecting xylooligosaccharides (XOS) production. Characterization of oil palm frond bagasse (OPFB), oil palm empty fruit bunch (OPEFB), and rice straw (RS) before and after pretreatment was performed in terms of chemical and physical properties. Enzymatic hydrolysis using a 25−1 fractional factorial design was further conducted to evaluate the effect of solid loading, enzyme loading, temperature, hydrolysis time and agitation on XOS production from the selected agricultural waste. From characterization results OPFB was determined as the greatest promising agricultural waste for XOS production. Pretreatment on OPFB simultaneously exhibited the highest increase of xylan recovery and lignin removal. XOS production up to 174.74 mg/g through enzymatic hydrolysis was successfully achieved from the pretreated OPFB. The enzymatic hydrolysis conditions of 1% solid loading and 50 U/mL enzyme loading at 55 °C temperature for 4 h hydrolysis time with 50 rpm of agitation were found efficient to obtain maximum XOS production. Among those factors solid loading was determined as the most significant factor that contributed to the increase of XOS production. The findings from this study have verified that acid pretreatment could improve the biomass structure by disrupting its structure which is important to facilitate enzymatic hydrolysis for high yield of XOS production.

KW - Acid pretreatment

KW - Enzymatic hydrolysis

KW - Oil palm frond bagasse

KW - Xylan recovery

KW - Xylooligosaccharides

UR - http://www.scopus.com/inward/record.url?scp=85058680244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058680244&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2018.12.042

DO - 10.1016/j.indcrop.2018.12.042

M3 - Article

AN - SCOPUS:85058680244

VL - 129

SP - 575

EP - 584

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -