Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors.

Original languageEnglish
Pages (from-to)1827-1833
Number of pages7
JournalSains Malaysiana
Volume43
Issue number12
Publication statusPublished - 1 Dec 2014

Fingerprint

Primulaceae
xanthine oxidase
extracts
methylene chloride
inhibitory concentration 50
antioxidant activity
allopurinol
xanthine
hypoxanthine
2,2-diphenyl-1-picrylhydrazyl
uric acid
plant anatomy
antioxidants
metabolism
assays

Keywords

  • Labisia pumila var. alata
  • Primulaceae
  • Total phenolic content
  • Xanthine oxidase inhibition

ASJC Scopus subject areas

  • General

Cite this

Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species. / Mamat, Norlida; Jamal, Jamia Azdina; Jantan, Ibrahim; Husain, Khairana.

In: Sains Malaysiana, Vol. 43, No. 12, 01.12.2014, p. 1827-1833.

Research output: Contribution to journalArticle

@article{a40479fbb4d64d5d8c31a216b46f0910,
title = "Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species",
abstract = "Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors.",
keywords = "Labisia pumila var. alata, Primulaceae, Total phenolic content, Xanthine oxidase inhibition",
author = "Norlida Mamat and Jamal, {Jamia Azdina} and Ibrahim Jantan and Khairana Husain",
year = "2014",
month = "12",
day = "1",
language = "English",
volume = "43",
pages = "1827--1833",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "12",

}

TY - JOUR

T1 - Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species

AU - Mamat, Norlida

AU - Jamal, Jamia Azdina

AU - Jantan, Ibrahim

AU - Husain, Khairana

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors.

AB - Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors.

KW - Labisia pumila var. alata

KW - Primulaceae

KW - Total phenolic content

KW - Xanthine oxidase inhibition

UR - http://www.scopus.com/inward/record.url?scp=84920181909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920181909&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84920181909

VL - 43

SP - 1827

EP - 1833

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 12

ER -