Wound healing potential of total sulfated glycosaminoglycan (GAG) from malaysian sea cucumber, Stichopus vastus coelomic fluid

Siti Fathiah Masre, George W. Yip, K. N S Sirajudeen, Farid Che Ghazali

Research output: Contribution to journalArticle

Abstract

Massive nutritional and medicinal values of sea cucumbers have long been reported in the past that may be due to sulfated Glycosaminoglycan (GAG). Thus, the effects of total sulfated GAG from local Stichopus vastus coelomic fluid were analyzed for its potential in full-fhickness excisional wound healing. The 20 uL of 1 jag mL-1 total sulfated GAG was applied topically to 6 mm diameter of full thickness wound from day 0-12 on the dorsal part of female sprague-dawley rats (n = 10). Whilst control group was treated with 20 uL of normal saline. Wound healing activities were assessed through wound contraction rate (%) and semi-quantitative measurement on the histology analysis of epithelial migration, inflammatory responses, fibroblasts proliferation, new vessels formation and collagen organization. Results showed significantly improve wound contraction rate on each observation day in the treatment group compared to the control group. Total sulfated GAG from S. vastus coelomic fluid showed significantly acceleration on wound healing process in epithelial migration, fibroblasts proliferation, new vessels formation and collagen organization. Data strongly documented the wound healing effects of sulfated GAG from the local sea cucumber S. vastus coelomic fluid.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalResearch Journal of Medical Sciences
Volume9
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

A73025
Stichopus
Sea Cucumbers
Wound Healing
Wounds and Injuries
Collagen
Fibroblasts
Control Groups
Nutritive Value
Sprague Dawley Rats
Histology
Observation

Keywords

  • Glycosaminoglycan
  • S. vastus
  • Sea cucumber
  • Sulfated GAG
  • Wound healing

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wound healing potential of total sulfated glycosaminoglycan (GAG) from malaysian sea cucumber, Stichopus vastus coelomic fluid. / Masre, Siti Fathiah; Yip, George W.; Sirajudeen, K. N S; Ghazali, Farid Che.

In: Research Journal of Medical Sciences, Vol. 9, No. 3, 2015, p. 67-72.

Research output: Contribution to journalArticle

@article{8fa1130970814d188a238b86bee13da8,
title = "Wound healing potential of total sulfated glycosaminoglycan (GAG) from malaysian sea cucumber, Stichopus vastus coelomic fluid",
abstract = "Massive nutritional and medicinal values of sea cucumbers have long been reported in the past that may be due to sulfated Glycosaminoglycan (GAG). Thus, the effects of total sulfated GAG from local Stichopus vastus coelomic fluid were analyzed for its potential in full-fhickness excisional wound healing. The 20 uL of 1 jag mL-1 total sulfated GAG was applied topically to 6 mm diameter of full thickness wound from day 0-12 on the dorsal part of female sprague-dawley rats (n = 10). Whilst control group was treated with 20 uL of normal saline. Wound healing activities were assessed through wound contraction rate ({\%}) and semi-quantitative measurement on the histology analysis of epithelial migration, inflammatory responses, fibroblasts proliferation, new vessels formation and collagen organization. Results showed significantly improve wound contraction rate on each observation day in the treatment group compared to the control group. Total sulfated GAG from S. vastus coelomic fluid showed significantly acceleration on wound healing process in epithelial migration, fibroblasts proliferation, new vessels formation and collagen organization. Data strongly documented the wound healing effects of sulfated GAG from the local sea cucumber S. vastus coelomic fluid.",
keywords = "Glycosaminoglycan, S. vastus, Sea cucumber, Sulfated GAG, Wound healing",
author = "Masre, {Siti Fathiah} and Yip, {George W.} and Sirajudeen, {K. N S} and Ghazali, {Farid Che}",
year = "2015",
doi = "10.3923/rjmsci.2015.67.72",
language = "English",
volume = "9",
pages = "67--72",
journal = "Research Journal of Medical Sciences",
issn = "1815-9346",
publisher = "Medwell Publishing",
number = "3",

}

TY - JOUR

T1 - Wound healing potential of total sulfated glycosaminoglycan (GAG) from malaysian sea cucumber, Stichopus vastus coelomic fluid

AU - Masre, Siti Fathiah

AU - Yip, George W.

AU - Sirajudeen, K. N S

AU - Ghazali, Farid Che

PY - 2015

Y1 - 2015

N2 - Massive nutritional and medicinal values of sea cucumbers have long been reported in the past that may be due to sulfated Glycosaminoglycan (GAG). Thus, the effects of total sulfated GAG from local Stichopus vastus coelomic fluid were analyzed for its potential in full-fhickness excisional wound healing. The 20 uL of 1 jag mL-1 total sulfated GAG was applied topically to 6 mm diameter of full thickness wound from day 0-12 on the dorsal part of female sprague-dawley rats (n = 10). Whilst control group was treated with 20 uL of normal saline. Wound healing activities were assessed through wound contraction rate (%) and semi-quantitative measurement on the histology analysis of epithelial migration, inflammatory responses, fibroblasts proliferation, new vessels formation and collagen organization. Results showed significantly improve wound contraction rate on each observation day in the treatment group compared to the control group. Total sulfated GAG from S. vastus coelomic fluid showed significantly acceleration on wound healing process in epithelial migration, fibroblasts proliferation, new vessels formation and collagen organization. Data strongly documented the wound healing effects of sulfated GAG from the local sea cucumber S. vastus coelomic fluid.

AB - Massive nutritional and medicinal values of sea cucumbers have long been reported in the past that may be due to sulfated Glycosaminoglycan (GAG). Thus, the effects of total sulfated GAG from local Stichopus vastus coelomic fluid were analyzed for its potential in full-fhickness excisional wound healing. The 20 uL of 1 jag mL-1 total sulfated GAG was applied topically to 6 mm diameter of full thickness wound from day 0-12 on the dorsal part of female sprague-dawley rats (n = 10). Whilst control group was treated with 20 uL of normal saline. Wound healing activities were assessed through wound contraction rate (%) and semi-quantitative measurement on the histology analysis of epithelial migration, inflammatory responses, fibroblasts proliferation, new vessels formation and collagen organization. Results showed significantly improve wound contraction rate on each observation day in the treatment group compared to the control group. Total sulfated GAG from S. vastus coelomic fluid showed significantly acceleration on wound healing process in epithelial migration, fibroblasts proliferation, new vessels formation and collagen organization. Data strongly documented the wound healing effects of sulfated GAG from the local sea cucumber S. vastus coelomic fluid.

KW - Glycosaminoglycan

KW - S. vastus

KW - Sea cucumber

KW - Sulfated GAG

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84947582728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947582728&partnerID=8YFLogxK

U2 - 10.3923/rjmsci.2015.67.72

DO - 10.3923/rjmsci.2015.67.72

M3 - Article

AN - SCOPUS:84947582728

VL - 9

SP - 67

EP - 72

JO - Research Journal of Medical Sciences

JF - Research Journal of Medical Sciences

SN - 1815-9346

IS - 3

ER -