Wintergreen oil

A novel method in Wheatley's trichrome staining technique

Fatmah Md Salleh, Tengku Shahrul Anuar, `Azlin Muhammad @ Mohd Yasin, Norhayati Moktar

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique, as the reference technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.

Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalJournal of Microbiological Methods
Volume91
Issue number1
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Xylenes
Staining and Labeling
Polyvinyl Alcohol
Parasitology
Poisons
Parasites
wintergreen oil
Oil A

Keywords

  • Staining technique
  • Wheatley's trichrome
  • Wintergreen oil

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Wintergreen oil : A novel method in Wheatley's trichrome staining technique. / Salleh, Fatmah Md; Anuar, Tengku Shahrul; Muhammad @ Mohd Yasin, `Azlin; Moktar, Norhayati.

In: Journal of Microbiological Methods, Vol. 91, No. 1, 10.2012, p. 174-178.

Research output: Contribution to journalArticle

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