Whole cell biosensor using Anabaena torulosa with optical transduction for environmental toxicity evaluation

Ling Shing Wong, Yook Heng Lee, Salmijah Surif

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A whole cell-based biosensor using Anabaena torulosa for the detection of heavy metals (Cu, Pb, and Cd), 2,4-dichlorophenoxyacetate (2,4-D), and chlorpyrifos was constructed. The cyanobacteria were entrapped on a cellulose membrane through filtration. Then, the membrane was dried and fixed into a cylindrical well, which was designed to be attached to an optical probe. The probe was connected to fluorescence spectrometer with optical fibre. The presence of the toxicants was indicated by the change of fluorescence emission, before and after the exposure. The linear detection ranges for Cu, Pb, and Cd were 2.5-10.0 μg/L, 0.5-5.0 μg/L, and 0.5-10.0 μg/L, respectively, while 2,4-D and chlorpyrifos shared similar linear ranges of 0.05-0.75 μg/L. The biosensor showed good sensitivity with the lowest limits of detection (LLD) for Cu, Pb, Cd, 2,4-D and chlorpyrifos determined at 1.195 μg/L, 0.100 μg/L, 0.027 μg/L, 0.025 μg/L, and 0.025 μg/L, respectively. The overall reproducibility of the biosensor (n=3) was <±6.35%. The biosensor had been tested with different combinations of toxicants, with the results showing predominantly antagonistic responses. The results confirmed that the biosensor constructed in this report is suitable to be used in quantitative and qualitative detections of heavy metals and pesticides.

Original languageEnglish
Article number567272
JournalJournal of Sensors
Volume2013
DOIs
Publication statusPublished - 2013

Fingerprint

anabaena
bioinstrumentation
Biosensors
toxicity
Toxicity
evaluation
cells
heavy metals
Heavy metals
Fluorescence
membranes
Membranes
fluorescence
pesticides
probes
Pesticides
cellulose
Spectrometers
Optical fibers
Cellulose

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

Whole cell biosensor using Anabaena torulosa with optical transduction for environmental toxicity evaluation. / Wong, Ling Shing; Lee, Yook Heng; Surif, Salmijah.

In: Journal of Sensors, Vol. 2013, 567272, 2013.

Research output: Contribution to journalArticle

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