Volatolomics combined terahertz time domain spectral analyses of colon cancer in vitro

Ahmad Zulhilmi Arshad, Yusof Munajat, Sib Krishna Ghoshal, Rahman Jamal, Nor Adzimah Johdi, Raja Kamarulzaman Raja Ibrahim, Hanif Zulkhairi

Research output: Contribution to journalArticle

Abstract

Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THzTDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GCMSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalJurnal Teknologi
Volume81
Issue number5
DOIs
Publication statusPublished - Sep 2019

Fingerprint

Cells
Spectroscopy
Volatile organic compounds
Gas chromatography
Principal component analysis
Spectrum analysis
Gases

Keywords

  • Chromatography
  • Colon cancer
  • Gas analysis
  • Terahertz spectra
  • Volatolomics analysis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Volatolomics combined terahertz time domain spectral analyses of colon cancer in vitro. / Arshad, Ahmad Zulhilmi; Munajat, Yusof; Ghoshal, Sib Krishna; Jamal, Rahman; Johdi, Nor Adzimah; Ibrahim, Raja Kamarulzaman Raja; Zulkhairi, Hanif.

In: Jurnal Teknologi, Vol. 81, No. 5, 09.2019, p. 105-112.

Research output: Contribution to journalArticle

Arshad, Ahmad Zulhilmi ; Munajat, Yusof ; Ghoshal, Sib Krishna ; Jamal, Rahman ; Johdi, Nor Adzimah ; Ibrahim, Raja Kamarulzaman Raja ; Zulkhairi, Hanif. / Volatolomics combined terahertz time domain spectral analyses of colon cancer in vitro. In: Jurnal Teknologi. 2019 ; Vol. 81, No. 5. pp. 105-112.
@article{23fe08a782f9472fb54b129840567cd8,
title = "Volatolomics combined terahertz time domain spectral analyses of colon cancer in vitro",
abstract = "Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THzTDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GCMSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.",
keywords = "Chromatography, Colon cancer, Gas analysis, Terahertz spectra, Volatolomics analysis",
author = "Arshad, {Ahmad Zulhilmi} and Yusof Munajat and Ghoshal, {Sib Krishna} and Rahman Jamal and Johdi, {Nor Adzimah} and Ibrahim, {Raja Kamarulzaman Raja} and Hanif Zulkhairi",
year = "2019",
month = "9",
doi = "10.11113/jt.v81.13310",
language = "English",
volume = "81",
pages = "105--112",
journal = "Jurnal Teknologi",
issn = "0127-9696",
publisher = "Penerbit Universiti Teknologi Malaysia",
number = "5",

}

TY - JOUR

T1 - Volatolomics combined terahertz time domain spectral analyses of colon cancer in vitro

AU - Arshad, Ahmad Zulhilmi

AU - Munajat, Yusof

AU - Ghoshal, Sib Krishna

AU - Jamal, Rahman

AU - Johdi, Nor Adzimah

AU - Ibrahim, Raja Kamarulzaman Raja

AU - Zulkhairi, Hanif

PY - 2019/9

Y1 - 2019/9

N2 - Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THzTDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GCMSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.

AB - Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THzTDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GCMSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.

KW - Chromatography

KW - Colon cancer

KW - Gas analysis

KW - Terahertz spectra

KW - Volatolomics analysis

UR - http://www.scopus.com/inward/record.url?scp=85073382862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073382862&partnerID=8YFLogxK

U2 - 10.11113/jt.v81.13310

DO - 10.11113/jt.v81.13310

M3 - Article

AN - SCOPUS:85073382862

VL - 81

SP - 105

EP - 112

JO - Jurnal Teknologi

JF - Jurnal Teknologi

SN - 0127-9696

IS - 5

ER -