Vitamin E improved bone strength and bone minerals in male rats given alcohol

Syuhada Zakaria, Siti Zulaikha Mat-Husain, Kong Ying-Hwey, Kek Xin-Kai, Abdullah Mohd-Badawi, Nurul Amiza Abd-Ghani, Muhamad Arizi Aziz, Norazlina Mohamed

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective(s): Alcohol consumption induces oxidative stress on bone, which in turn increases the risk of osteoporosis. This study determined the effects of vitamin E on bone strength and bone mineral content in alcohol-induced osteoporotic rats. Materials and Methods: Three months old Sprague Dawley male rats were randomly divided into 5 groups: (I) control group; (II) alcohol (3 g/kg) + normal saline; (III) alcohol (3 g/kg) + olive oil; (IV) alcohol (3 g/kg) + alpha-tocopherol (60 mg/kg) and (V) alcohol (3 g/kg) + palm vitamin E (60 mg/kg). The treatment lasted for three months. Following sacrifice, the right tibia was subjected to bone biomechanical test while the lumbar (fourth and fifth lumbar) and left tibia bones were harvested for bone mineral measurement. Results: Alcohol caused reduction in bone biomechanical parameters (maximum force, ultimate stress, yield stress and Young’s modulus) and bone minerals (bone calcium and magnesium) compared to control group (P<0.05). Palm vitamin E was able to improve bone biomechanical parameters by increasing the maximum force, ultimate stress and Young’s modulus (P<0.05) while alpha-tocopherol was not able to. Both alpha-tocopherol and palm vitamin E were able to significantly increase tibia calcium and magnesium content while only alpha-tocopherol caused significant increase in lumbar calcium content (P<0.05). Conclusion: Both palm vitamin E and alpha-tocopherol improved bone mineral content which was reduced by alcohol. However, only palm vitamin E was able to improve bone strength in alcohol treated rats.

Original languageEnglish
Pages (from-to)1360-1367
Number of pages8
JournalIranian Journal of Basic Medical Sciences
Volume20
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Vitamin E
Minerals
Rats
Bone
Alcohols
Bone and Bones
alpha-Tocopherol
Tibia
Elastic Modulus
Calcium
Bone Density
Magnesium
Control Groups
Elastic moduli
Alcohol Drinking
Osteoporosis
Oxidative stress
Sprague Dawley Rats
Oxidative Stress
Yield stress

Keywords

  • Alcohol-induced disorder
  • Bone minerals
  • Bone strength
  • Palm oil
  • Vitamin E

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Immunology and Microbiology(all)
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Zakaria, S., Mat-Husain, S. Z., Ying-Hwey, K., Xin-Kai, K., Mohd-Badawi, A., Abd-Ghani, N. A., ... Mohamed, N. (2017). Vitamin E improved bone strength and bone minerals in male rats given alcohol. Iranian Journal of Basic Medical Sciences, 20(12), 1360-1367. https://doi.org/10.22038/ijbms.2017.9610

Vitamin E improved bone strength and bone minerals in male rats given alcohol. / Zakaria, Syuhada; Mat-Husain, Siti Zulaikha; Ying-Hwey, Kong; Xin-Kai, Kek; Mohd-Badawi, Abdullah; Abd-Ghani, Nurul Amiza; Aziz, Muhamad Arizi; Mohamed, Norazlina.

In: Iranian Journal of Basic Medical Sciences, Vol. 20, No. 12, 01.12.2017, p. 1360-1367.

Research output: Contribution to journalArticle

Zakaria, S, Mat-Husain, SZ, Ying-Hwey, K, Xin-Kai, K, Mohd-Badawi, A, Abd-Ghani, NA, Aziz, MA & Mohamed, N 2017, 'Vitamin E improved bone strength and bone minerals in male rats given alcohol', Iranian Journal of Basic Medical Sciences, vol. 20, no. 12, pp. 1360-1367. https://doi.org/10.22038/ijbms.2017.9610
Zakaria S, Mat-Husain SZ, Ying-Hwey K, Xin-Kai K, Mohd-Badawi A, Abd-Ghani NA et al. Vitamin E improved bone strength and bone minerals in male rats given alcohol. Iranian Journal of Basic Medical Sciences. 2017 Dec 1;20(12):1360-1367. https://doi.org/10.22038/ijbms.2017.9610
Zakaria, Syuhada ; Mat-Husain, Siti Zulaikha ; Ying-Hwey, Kong ; Xin-Kai, Kek ; Mohd-Badawi, Abdullah ; Abd-Ghani, Nurul Amiza ; Aziz, Muhamad Arizi ; Mohamed, Norazlina. / Vitamin E improved bone strength and bone minerals in male rats given alcohol. In: Iranian Journal of Basic Medical Sciences. 2017 ; Vol. 20, No. 12. pp. 1360-1367.
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abstract = "Objective(s): Alcohol consumption induces oxidative stress on bone, which in turn increases the risk of osteoporosis. This study determined the effects of vitamin E on bone strength and bone mineral content in alcohol-induced osteoporotic rats. Materials and Methods: Three months old Sprague Dawley male rats were randomly divided into 5 groups: (I) control group; (II) alcohol (3 g/kg) + normal saline; (III) alcohol (3 g/kg) + olive oil; (IV) alcohol (3 g/kg) + alpha-tocopherol (60 mg/kg) and (V) alcohol (3 g/kg) + palm vitamin E (60 mg/kg). The treatment lasted for three months. Following sacrifice, the right tibia was subjected to bone biomechanical test while the lumbar (fourth and fifth lumbar) and left tibia bones were harvested for bone mineral measurement. Results: Alcohol caused reduction in bone biomechanical parameters (maximum force, ultimate stress, yield stress and Young’s modulus) and bone minerals (bone calcium and magnesium) compared to control group (P<0.05). Palm vitamin E was able to improve bone biomechanical parameters by increasing the maximum force, ultimate stress and Young’s modulus (P<0.05) while alpha-tocopherol was not able to. Both alpha-tocopherol and palm vitamin E were able to significantly increase tibia calcium and magnesium content while only alpha-tocopherol caused significant increase in lumbar calcium content (P<0.05). Conclusion: Both palm vitamin E and alpha-tocopherol improved bone mineral content which was reduced by alcohol. However, only palm vitamin E was able to improve bone strength in alcohol treated rats.",
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AB - Objective(s): Alcohol consumption induces oxidative stress on bone, which in turn increases the risk of osteoporosis. This study determined the effects of vitamin E on bone strength and bone mineral content in alcohol-induced osteoporotic rats. Materials and Methods: Three months old Sprague Dawley male rats were randomly divided into 5 groups: (I) control group; (II) alcohol (3 g/kg) + normal saline; (III) alcohol (3 g/kg) + olive oil; (IV) alcohol (3 g/kg) + alpha-tocopherol (60 mg/kg) and (V) alcohol (3 g/kg) + palm vitamin E (60 mg/kg). The treatment lasted for three months. Following sacrifice, the right tibia was subjected to bone biomechanical test while the lumbar (fourth and fifth lumbar) and left tibia bones were harvested for bone mineral measurement. Results: Alcohol caused reduction in bone biomechanical parameters (maximum force, ultimate stress, yield stress and Young’s modulus) and bone minerals (bone calcium and magnesium) compared to control group (P<0.05). Palm vitamin E was able to improve bone biomechanical parameters by increasing the maximum force, ultimate stress and Young’s modulus (P<0.05) while alpha-tocopherol was not able to. Both alpha-tocopherol and palm vitamin E were able to significantly increase tibia calcium and magnesium content while only alpha-tocopherol caused significant increase in lumbar calcium content (P<0.05). Conclusion: Both palm vitamin E and alpha-tocopherol improved bone mineral content which was reduced by alcohol. However, only palm vitamin E was able to improve bone strength in alcohol treated rats.

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