Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens

F. B. Ong, W. Z. Wan Ngah, A. G M Top, B. A K Khaud, N. A. Shamaan

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    1. 1. The effects of α-tocopherol and γ-tocotrienol on glutathione S-transferase (GST) and γ-glutamyl transpeptidase (γ-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. 2. Both the α-tocopherol and γ-tocotrienol treated hepatocytes showed significantly higher (P < 0.05) GST activities than untreated hepatocytes prepared from the carcinogen treated rats in the first 3 days of culture. Treatment with α-tocopherol and γ-tocotrienol generally resulted in a tendency to increase the GST activities above that in the untreated hepatocytes. 3. 3. Treatment with high doses (125-250 μM) of α-tocopherol and low doses (12.5-25 μM) of γ-tocotrienol generally resulted in a significant reduction in γ-GT activities at 1-3 days. γ-GT activities are reduced as the dose of α-tocopherol and γ-tocotrienol are increased.

    Original languageEnglish
    Pages (from-to)397-402
    Number of pages6
    JournalInternational Journal of Biochemistry
    Volume26
    Issue number3
    DOIs
    Publication statusPublished - 1994

    Fingerprint

    Tocotrienols
    Tocopherols
    gamma-Glutamyltransferase
    Glutathione Transferase
    Vitamin E
    Carcinogens
    Rats
    Hepatocytes
    2-Acetylaminofluorene
    Diethylnitrosamine

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens. / Ong, F. B.; Wan Ngah, W. Z.; Top, A. G M; Khaud, B. A K; Shamaan, N. A.

    In: International Journal of Biochemistry, Vol. 26, No. 3, 1994, p. 397-402.

    Research output: Contribution to journalArticle

    Ong, F. B. ; Wan Ngah, W. Z. ; Top, A. G M ; Khaud, B. A K ; Shamaan, N. A. / Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens. In: International Journal of Biochemistry. 1994 ; Vol. 26, No. 3. pp. 397-402.
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    AU - Wan Ngah, W. Z.

    AU - Top, A. G M

    AU - Khaud, B. A K

    AU - Shamaan, N. A.

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    N2 - 1. 1. The effects of α-tocopherol and γ-tocotrienol on glutathione S-transferase (GST) and γ-glutamyl transpeptidase (γ-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. 2. Both the α-tocopherol and γ-tocotrienol treated hepatocytes showed significantly higher (P < 0.05) GST activities than untreated hepatocytes prepared from the carcinogen treated rats in the first 3 days of culture. Treatment with α-tocopherol and γ-tocotrienol generally resulted in a tendency to increase the GST activities above that in the untreated hepatocytes. 3. 3. Treatment with high doses (125-250 μM) of α-tocopherol and low doses (12.5-25 μM) of γ-tocotrienol generally resulted in a significant reduction in γ-GT activities at 1-3 days. γ-GT activities are reduced as the dose of α-tocopherol and γ-tocotrienol are increased.

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