Kesan polifenol minyak kelapa dara ke atas aktiviti enzim antioksidan dan lipid peroksida pada sel osteoblas

Translated title of the contribution: Virgin coconut oil polyphenol effects on antioxidant enzyme activities and lipid peroxidation on osteoblast cell

Research output: Contribution to journalArticle

Abstract

Osteoporosis is associated with free radicals and oxidative stress. The high polyphenol contents in virgin coconut oil (VCO) can prevent oxidative stress and consequently osteoporosis. This in vitro study was conducted in order to determine the mechanism of the protective effects of polyphenol extracted from VCO on murine osteoblast-like cell line (MC3T3-E1) which was induced with oxidative stress. To study the protective effects on viability of MC3T3-E1 cells exposed to hydrogen peroxide (H2O2) toxicity, MC3T3-E1 cells were post-treated with different doses of polyphenol for 24 h after 1 h incubation with 250 µM of H2O2 (IC50). The protective mechanism was studied by measuring melondialdehyde (MDA) levels as well as antioxidant enzymes activities which are superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). The results showed that the median inhibitary concentration (IC50) for H2O2 after 1 h was 250 µM. Cell viability tests showed that all the doses of polyphenol were able to protect MC3T3-E1 cells from H2O2 toxicity. The dose of 1 and 0.01 µM of polyphenol increased the level of GPx and CAT while the dose of 0.01 and 0.0001 µM increased the level of SOD. Meanwhile, the 0.0001 µM of PF prevented the MDA elevation. In conclucion, the polyphenol components in virgin coconut oil prevent lipid peroxidation in MC3T3-E1 cells induced with H2O2 by increasing the endogenous antioxidant enzymes.

Original languageMalay
Pages (from-to)2401-2409
Number of pages9
JournalSains Malaysiana
Volume47
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

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coconut oil
osteoblasts
polyphenols
lipid peroxidation
enzyme activity
antioxidants
oxidative stress
osteoporosis
cells
dosage
glutathione peroxidase
protective effect
inhibitory concentration 50
catalase
superoxide dismutase
toxicity
in vitro studies
cell viability
hydrogen peroxide
cell lines

ASJC Scopus subject areas

  • General

Cite this

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title = "Kesan polifenol minyak kelapa dara ke atas aktiviti enzim antioksidan dan lipid peroksida pada sel osteoblas",
abstract = "Osteoporosis is associated with free radicals and oxidative stress. The high polyphenol contents in virgin coconut oil (VCO) can prevent oxidative stress and consequently osteoporosis. This in vitro study was conducted in order to determine the mechanism of the protective effects of polyphenol extracted from VCO on murine osteoblast-like cell line (MC3T3-E1) which was induced with oxidative stress. To study the protective effects on viability of MC3T3-E1 cells exposed to hydrogen peroxide (H2O2) toxicity, MC3T3-E1 cells were post-treated with different doses of polyphenol for 24 h after 1 h incubation with 250 µM of H2O2 (IC50). The protective mechanism was studied by measuring melondialdehyde (MDA) levels as well as antioxidant enzymes activities which are superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). The results showed that the median inhibitary concentration (IC50) for H2O2 after 1 h was 250 µM. Cell viability tests showed that all the doses of polyphenol were able to protect MC3T3-E1 cells from H2O2 toxicity. The dose of 1 and 0.01 µM of polyphenol increased the level of GPx and CAT while the dose of 0.01 and 0.0001 µM increased the level of SOD. Meanwhile, the 0.0001 µM of PF prevented the MDA elevation. In conclucion, the polyphenol components in virgin coconut oil prevent lipid peroxidation in MC3T3-E1 cells induced with H2O2 by increasing the endogenous antioxidant enzymes.",
keywords = "MC3T3-E1 cells, Osteoporosis, Oxidative stress, Polyphenol, VCO",
author = "Zil Hayatullina and Norazlina Mohamed and Soelaiman, {Ima Nirwana} and Norliza Muhammad",
year = "2018",
month = "10",
day = "1",
doi = "10.17576/jsm-2018-4710-16",
language = "Malay",
volume = "47",
pages = "2401--2409",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "10",

}

TY - JOUR

T1 - Kesan polifenol minyak kelapa dara ke atas aktiviti enzim antioksidan dan lipid peroksida pada sel osteoblas

AU - Hayatullina, Zil

AU - Mohamed, Norazlina

AU - Soelaiman, Ima Nirwana

AU - Muhammad, Norliza

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Osteoporosis is associated with free radicals and oxidative stress. The high polyphenol contents in virgin coconut oil (VCO) can prevent oxidative stress and consequently osteoporosis. This in vitro study was conducted in order to determine the mechanism of the protective effects of polyphenol extracted from VCO on murine osteoblast-like cell line (MC3T3-E1) which was induced with oxidative stress. To study the protective effects on viability of MC3T3-E1 cells exposed to hydrogen peroxide (H2O2) toxicity, MC3T3-E1 cells were post-treated with different doses of polyphenol for 24 h after 1 h incubation with 250 µM of H2O2 (IC50). The protective mechanism was studied by measuring melondialdehyde (MDA) levels as well as antioxidant enzymes activities which are superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). The results showed that the median inhibitary concentration (IC50) for H2O2 after 1 h was 250 µM. Cell viability tests showed that all the doses of polyphenol were able to protect MC3T3-E1 cells from H2O2 toxicity. The dose of 1 and 0.01 µM of polyphenol increased the level of GPx and CAT while the dose of 0.01 and 0.0001 µM increased the level of SOD. Meanwhile, the 0.0001 µM of PF prevented the MDA elevation. In conclucion, the polyphenol components in virgin coconut oil prevent lipid peroxidation in MC3T3-E1 cells induced with H2O2 by increasing the endogenous antioxidant enzymes.

AB - Osteoporosis is associated with free radicals and oxidative stress. The high polyphenol contents in virgin coconut oil (VCO) can prevent oxidative stress and consequently osteoporosis. This in vitro study was conducted in order to determine the mechanism of the protective effects of polyphenol extracted from VCO on murine osteoblast-like cell line (MC3T3-E1) which was induced with oxidative stress. To study the protective effects on viability of MC3T3-E1 cells exposed to hydrogen peroxide (H2O2) toxicity, MC3T3-E1 cells were post-treated with different doses of polyphenol for 24 h after 1 h incubation with 250 µM of H2O2 (IC50). The protective mechanism was studied by measuring melondialdehyde (MDA) levels as well as antioxidant enzymes activities which are superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). The results showed that the median inhibitary concentration (IC50) for H2O2 after 1 h was 250 µM. Cell viability tests showed that all the doses of polyphenol were able to protect MC3T3-E1 cells from H2O2 toxicity. The dose of 1 and 0.01 µM of polyphenol increased the level of GPx and CAT while the dose of 0.01 and 0.0001 µM increased the level of SOD. Meanwhile, the 0.0001 µM of PF prevented the MDA elevation. In conclucion, the polyphenol components in virgin coconut oil prevent lipid peroxidation in MC3T3-E1 cells induced with H2O2 by increasing the endogenous antioxidant enzymes.

KW - MC3T3-E1 cells

KW - Osteoporosis

KW - Oxidative stress

KW - Polyphenol

KW - VCO

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U2 - 10.17576/jsm-2018-4710-16

DO - 10.17576/jsm-2018-4710-16

M3 - Article

VL - 47

SP - 2401

EP - 2409

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 10

ER -