Viability of Acanthamoeba after exposure to multipurpose contact lens disinfecting solutions

Mohamed Kamel Abdul Ghani, Ee Chin Liew, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, Wan Omar Abdullah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Contact lens wear is a major risk factor for Acanthamoeba keratitis. Disinfection solutions effective at killing Acanthamoeba organisms are important in preventing corneal infection. This study was therefore performed to gain insight into how commercial contact lens disinfecting solutions affect cysts of a clinical isolate of Acanthamoeba castellani. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Boston SIMPLICITYTM, Opti-Free Express® and CompleteTM were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 4 to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Boston SIMPLICITYTM, Opti-Free Express® and CompleteTM) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers but Boston SIMPLICITYTM was effective at inactivating the cysts at 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. Boston SIMPLICITYTM showed its effectiveness when the exposure time is extended to 8 hours.

Original languageEnglish
Pages (from-to)509-511
Number of pages3
JournalInternational Medical Journal
Volume23
Issue number5
Publication statusPublished - 2016

Fingerprint

Contact Lens Solutions
Acanthamoeba
Cysts
Acanthamoeba Keratitis
Trophozoites
Disinfection
Contact Lenses
Agar
Suspensions
Escherichia coli
Growth
Infection

Keywords

  • Acanthamoeba
  • Malaysia
  • Multipurpose contact lens disinfecting solutions

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Viability of Acanthamoeba after exposure to multipurpose contact lens disinfecting solutions. / Abdul Ghani, Mohamed Kamel; Liew, Ee Chin; Nordin, Anisah; Suboh, Yusof; Ab Rahim, Noraina; Ahmad, Norazah; Abdullah, Wan Omar.

In: International Medical Journal, Vol. 23, No. 5, 2016, p. 509-511.

Research output: Contribution to journalArticle

Abdul Ghani, MK, Liew, EC, Nordin, A, Suboh, Y, Ab Rahim, N, Ahmad, N & Abdullah, WO 2016, 'Viability of Acanthamoeba after exposure to multipurpose contact lens disinfecting solutions', International Medical Journal, vol. 23, no. 5, pp. 509-511.
Abdul Ghani, Mohamed Kamel ; Liew, Ee Chin ; Nordin, Anisah ; Suboh, Yusof ; Ab Rahim, Noraina ; Ahmad, Norazah ; Abdullah, Wan Omar. / Viability of Acanthamoeba after exposure to multipurpose contact lens disinfecting solutions. In: International Medical Journal. 2016 ; Vol. 23, No. 5. pp. 509-511.
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