Viability and apoptotic effects of green tea (camellia sinensis) methanol extract on human leukemic cell lines

Research output: Contribution to journalArticle

Abstract

Apoptosis or programmed cell death is a highly regulated and controlled process that confers advantages during an organismsi lifecycle. Compounds which can induce apoptosis in leukemic cells are worthy to be developed as new antileukemic agents. The objective of this study was to investigate the cytotoxicity and apoptotic effects of methanol extract of Camellia sinensis or universally known as green teaon U-937, Jurkat Clone E6-1 and K-562 cell lines. The cells were treated with the extract at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 g/mL and their viability were determined at 48 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. The mode of cell death was determined using a flow cytometry. Our results showed that the half maximal inhibitory concentrations (IC50) of C. Sinensis on U-937, Jurkat Clone E6-1 and K-562 cell lines were 88 } 1.89, 98 } 1.96 and 205 } 2.23 g/mL, respectively. Cells undergoing apoptosis were significantly higher than the negative controlwith a percentage of 41.93 } 2.98 % in U-937 cells (p < 0.001), 29.53 } 0.34% in Jurkat Clone E6-1 cells (p < 0.01) and 33.27 } 3.43% in K-562 cells (p < 0.001). However, necrosis values were significantly higher than negative control in U-937 cells (23.90 } 1.25%, p < 0.001) and Jurkat Clone E6-1 cells (18.93 } 0.96%, p < 0.001). In conclusion, green tea extract has a potential as an inducer of apoptosis in all the leukemic cells tested.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalActa Poloniae Pharmaceutica - Drug Research
Volume75
Issue number1
Publication statusPublished - 1 Jan 2018

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Camellia sinensis
Tea
Methanol
Cell Line
Clone Cells
Apoptosis
Cell Death
Inhibitory Concentration 50
Flow Cytometry
Necrosis

Keywords

  • Apoptotic effect
  • Camellia sinensis
  • Cell viability
  • Green tea
  • Human leukemic cells

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{80588bdb13f7418fa74e80cfd230fc2f,
title = "Viability and apoptotic effects of green tea (camellia sinensis) methanol extract on human leukemic cell lines",
abstract = "Apoptosis or programmed cell death is a highly regulated and controlled process that confers advantages during an organismsi lifecycle. Compounds which can induce apoptosis in leukemic cells are worthy to be developed as new antileukemic agents. The objective of this study was to investigate the cytotoxicity and apoptotic effects of methanol extract of Camellia sinensis or universally known as green teaon U-937, Jurkat Clone E6-1 and K-562 cell lines. The cells were treated with the extract at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 g/mL and their viability were determined at 48 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. The mode of cell death was determined using a flow cytometry. Our results showed that the half maximal inhibitory concentrations (IC50) of C. Sinensis on U-937, Jurkat Clone E6-1 and K-562 cell lines were 88 } 1.89, 98 } 1.96 and 205 } 2.23 g/mL, respectively. Cells undergoing apoptosis were significantly higher than the negative controlwith a percentage of 41.93 } 2.98 {\%} in U-937 cells (p < 0.001), 29.53 } 0.34{\%} in Jurkat Clone E6-1 cells (p < 0.01) and 33.27 } 3.43{\%} in K-562 cells (p < 0.001). However, necrosis values were significantly higher than negative control in U-937 cells (23.90 } 1.25{\%}, p < 0.001) and Jurkat Clone E6-1 cells (18.93 } 0.96{\%}, p < 0.001). In conclusion, green tea extract has a potential as an inducer of apoptosis in all the leukemic cells tested.",
keywords = "Apoptotic effect, Camellia sinensis, Cell viability, Green tea, Human leukemic cells",
author = "Norazrina Azmi and Hui, {Chee Sze} and {Mohd Fauzi}, Norsyahida and Malina Jasamai and Endang, {Kumolosasi Msi}",
year = "2018",
month = "1",
day = "1",
language = "English",
volume = "75",
pages = "51--58",
journal = "Acta Poloniae Pharmaceutica",
issn = "0001-6837",
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TY - JOUR

T1 - Viability and apoptotic effects of green tea (camellia sinensis) methanol extract on human leukemic cell lines

AU - Azmi, Norazrina

AU - Hui, Chee Sze

AU - Mohd Fauzi, Norsyahida

AU - Jasamai, Malina

AU - Endang, Kumolosasi Msi

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Apoptosis or programmed cell death is a highly regulated and controlled process that confers advantages during an organismsi lifecycle. Compounds which can induce apoptosis in leukemic cells are worthy to be developed as new antileukemic agents. The objective of this study was to investigate the cytotoxicity and apoptotic effects of methanol extract of Camellia sinensis or universally known as green teaon U-937, Jurkat Clone E6-1 and K-562 cell lines. The cells were treated with the extract at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 g/mL and their viability were determined at 48 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. The mode of cell death was determined using a flow cytometry. Our results showed that the half maximal inhibitory concentrations (IC50) of C. Sinensis on U-937, Jurkat Clone E6-1 and K-562 cell lines were 88 } 1.89, 98 } 1.96 and 205 } 2.23 g/mL, respectively. Cells undergoing apoptosis were significantly higher than the negative controlwith a percentage of 41.93 } 2.98 % in U-937 cells (p < 0.001), 29.53 } 0.34% in Jurkat Clone E6-1 cells (p < 0.01) and 33.27 } 3.43% in K-562 cells (p < 0.001). However, necrosis values were significantly higher than negative control in U-937 cells (23.90 } 1.25%, p < 0.001) and Jurkat Clone E6-1 cells (18.93 } 0.96%, p < 0.001). In conclusion, green tea extract has a potential as an inducer of apoptosis in all the leukemic cells tested.

AB - Apoptosis or programmed cell death is a highly regulated and controlled process that confers advantages during an organismsi lifecycle. Compounds which can induce apoptosis in leukemic cells are worthy to be developed as new antileukemic agents. The objective of this study was to investigate the cytotoxicity and apoptotic effects of methanol extract of Camellia sinensis or universally known as green teaon U-937, Jurkat Clone E6-1 and K-562 cell lines. The cells were treated with the extract at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 g/mL and their viability were determined at 48 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. The mode of cell death was determined using a flow cytometry. Our results showed that the half maximal inhibitory concentrations (IC50) of C. Sinensis on U-937, Jurkat Clone E6-1 and K-562 cell lines were 88 } 1.89, 98 } 1.96 and 205 } 2.23 g/mL, respectively. Cells undergoing apoptosis were significantly higher than the negative controlwith a percentage of 41.93 } 2.98 % in U-937 cells (p < 0.001), 29.53 } 0.34% in Jurkat Clone E6-1 cells (p < 0.01) and 33.27 } 3.43% in K-562 cells (p < 0.001). However, necrosis values were significantly higher than negative control in U-937 cells (23.90 } 1.25%, p < 0.001) and Jurkat Clone E6-1 cells (18.93 } 0.96%, p < 0.001). In conclusion, green tea extract has a potential as an inducer of apoptosis in all the leukemic cells tested.

KW - Apoptotic effect

KW - Camellia sinensis

KW - Cell viability

KW - Green tea

KW - Human leukemic cells

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