VEGF Polymorphisms among Neovascular Age-Related Macular Degenerative Subjects in a Multiethnic Population

Nur Afiqah Mohamad, Vasudevan Ramachandran, Patimah Ismail, Hazlita Mohd Isa, Yoke Mun Chan, Nor Fariza Ngah, Norshakimah Md Bakri, Siew Mooi Ching, Fan Kee Hoo, Wan Aliaa Wan Sulaiman

Research output: Contribution to journalArticle

Abstract

Aim: To determine the association of vascular endothelial growth factor (VEGF) polymorphisms with neovascular age-related macular degeneration (nAMD). Materials and Methods: One hundred thirty-five nAMD patients and 135 controls were recruited to determine the association of the -460 C/T, the -2549 I/D, and the +405 G/C polymorphisms with the VEGF gene. Genotyping was conducted using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach, and association analyses were conducted using chi-square analysis and logistic regression analysis. Results: A significant association was observed between nAMD and the VEGF +405 G/C genotypes (p = 0.002) and alleles (odds ratio = 1.36, 95% confidence interval = 1.12-1.62, p = < 0.001) compared with the controls. This association was confirmed by logistic regression analyses using two different genetic models (additive and dominant) resulting in p-values of p = 0.001 and p < 0.001, respectively. In addition, the dominant model of VEGF +405 G/C was also found to be at risk of the CC genotype with nAMD among subjects that were aged ≥60 years, female, of Chinese ethnicity, hypertensive, diabetic, and smokers. Conclusion: With the exception of several limitations, the present study showed evidence of an association between the VEGF +405 G/C polymorphism and nAMD in Malaysian subjects.

Original languageEnglish
Pages (from-to)600-607
Number of pages8
JournalGenetic Testing and Molecular Biomarkers
Volume21
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

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Macular Degeneration
Vascular Endothelial Growth Factor A
Population
Logistic Models
Genotype
Regression Analysis
Genetic Models
Restriction Fragment Length Polymorphisms
Alleles
Odds Ratio
Confidence Intervals
Polymerase Chain Reaction
Genes

Keywords

  • age-related macular degeneration
  • polymorphism
  • vascular endothelial growth factor

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

VEGF Polymorphisms among Neovascular Age-Related Macular Degenerative Subjects in a Multiethnic Population. / Mohamad, Nur Afiqah; Ramachandran, Vasudevan; Ismail, Patimah; Mohd Isa, Hazlita; Chan, Yoke Mun; Ngah, Nor Fariza; Md Bakri, Norshakimah; Ching, Siew Mooi; Hoo, Fan Kee; Wan Sulaiman, Wan Aliaa.

In: Genetic Testing and Molecular Biomarkers, Vol. 21, No. 10, 01.10.2017, p. 600-607.

Research output: Contribution to journalArticle

Mohamad, NA, Ramachandran, V, Ismail, P, Mohd Isa, H, Chan, YM, Ngah, NF, Md Bakri, N, Ching, SM, Hoo, FK & Wan Sulaiman, WA 2017, 'VEGF Polymorphisms among Neovascular Age-Related Macular Degenerative Subjects in a Multiethnic Population', Genetic Testing and Molecular Biomarkers, vol. 21, no. 10, pp. 600-607. https://doi.org/10.1089/gtmb.2017.0079
Mohamad, Nur Afiqah ; Ramachandran, Vasudevan ; Ismail, Patimah ; Mohd Isa, Hazlita ; Chan, Yoke Mun ; Ngah, Nor Fariza ; Md Bakri, Norshakimah ; Ching, Siew Mooi ; Hoo, Fan Kee ; Wan Sulaiman, Wan Aliaa. / VEGF Polymorphisms among Neovascular Age-Related Macular Degenerative Subjects in a Multiethnic Population. In: Genetic Testing and Molecular Biomarkers. 2017 ; Vol. 21, No. 10. pp. 600-607.
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AU - Chan, Yoke Mun

AU - Ngah, Nor Fariza

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