Variations of huperzine A content in Lycopodiaceae species from tropics

NorShahidah Sahidan, Chee Yan Choo, A. Latiff, Razali Jaman

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    (-) Huperzine A is a bioactive alkaloid from Huperzia serrata (Lycopodiaceae) used for the treatment of Alzheimer's disease. High yielding (-) huperzine A species is of interest for mass propagation since it grows slowly in temperate countries. The content of (-) huperzine A from temperate countries was reported but none reported from tropical species. The aim of this study was to evaluate the content of (-) huperzine A from tropical club mosses and further identify a high yielding species. Club mosses from Lycopodiaceae family were collected from Peninsular Malaysia. The collected club mosses were dried, pulverized and extracted with methanol. A gradient reverse phase HPLC-photodiode array detector method with increasing amount of methanol in 0.01 % trifluoroacetic acid was developed. The calibration curve was linear from 5 to 100 μgmL -1 with correlation coefficient, r 2, of 0.998 1. The precision for both intra- and inter-day peak area were between 0.48% to 1.24 % and 0.95% to 3.85 %, respectively. The recovery of the method was between 99.8% to 103.8 %. Though geographically segregated, (-) huperzine A content in Huperzia phlegmaria and H.carinata found in the tropics was highest and similar to species in Australasia. Both species may provide a good source of (-) huperzine A.

    Original languageEnglish
    Pages (from-to)125-128
    Number of pages4
    JournalChinese Journal of Natural Medicines
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - Mar 2012

    Fingerprint

    Lycopodiaceae
    Huperzia
    Methanol
    Australasia
    Trifluoroacetic Acid
    Malaysia
    Alkaloids
    Calibration
    huperzine A
    Alzheimer Disease
    High Pressure Liquid Chromatography

    Keywords

    • (-) Huperzine A
    • HPLC
    • Lycopodiaceae
    • Tropics

    ASJC Scopus subject areas

    • Complementary and alternative medicine
    • Drug Discovery

    Cite this

    Variations of huperzine A content in Lycopodiaceae species from tropics. / Sahidan, NorShahidah; Choo, Chee Yan; Latiff, A.; Jaman, Razali.

    In: Chinese Journal of Natural Medicines, Vol. 10, No. 2, 03.2012, p. 125-128.

    Research output: Contribution to journalArticle

    Sahidan, NorShahidah ; Choo, Chee Yan ; Latiff, A. ; Jaman, Razali. / Variations of huperzine A content in Lycopodiaceae species from tropics. In: Chinese Journal of Natural Medicines. 2012 ; Vol. 10, No. 2. pp. 125-128.
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    abstract = "(-) Huperzine A is a bioactive alkaloid from Huperzia serrata (Lycopodiaceae) used for the treatment of Alzheimer's disease. High yielding (-) huperzine A species is of interest for mass propagation since it grows slowly in temperate countries. The content of (-) huperzine A from temperate countries was reported but none reported from tropical species. The aim of this study was to evaluate the content of (-) huperzine A from tropical club mosses and further identify a high yielding species. Club mosses from Lycopodiaceae family were collected from Peninsular Malaysia. The collected club mosses were dried, pulverized and extracted with methanol. A gradient reverse phase HPLC-photodiode array detector method with increasing amount of methanol in 0.01 {\%} trifluoroacetic acid was developed. The calibration curve was linear from 5 to 100 μgmL -1 with correlation coefficient, r 2, of 0.998 1. The precision for both intra- and inter-day peak area were between 0.48{\%} to 1.24 {\%} and 0.95{\%} to 3.85 {\%}, respectively. The recovery of the method was between 99.8{\%} to 103.8 {\%}. Though geographically segregated, (-) huperzine A content in Huperzia phlegmaria and H.carinata found in the tropics was highest and similar to species in Australasia. Both species may provide a good source of (-) huperzine A.",
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