Variations in mitochondrial DNA control region among Malay and Chinese subpopulations (sequence 16000–16200)

Seri Mirianti Ishar, Kiroobhashini Parameswaran, Nur Safrina Masduki, Rus Dina Rus Din

Research output: Contribution to journalArticle

Abstract

DNA variations are alterations found in DNA sequence, occurring in both nuclear DNA and mitochondrial DNA. Variations might differ in individual following population, respectively. The aim of this study was to find variations in target sequence of mtDNA (16000–16200) to be used as marker in Malay and Chinese population. A total of 30 buccal swab samples from 20 Malay and 10 Chinese subjects were collected and preserved on FTA card. The FTA card that contained DNA sample was punched to be included into polymerase chain reaction mixture. Amplification was carried out and the products were sequenced. Sequence variations were found in both Malay and Chinese populations. A total of nine variations (16129, 16108, 16162, 16172, 16148, 16127, 16173, 16099 and 16100) were found in Malay population while a total of seven variations (16129, 16104, 16111, 16109, 16164, 16170 and 16136) were found in Chinese population. Nucleotide position 16129 was found as variation in both Malay and Chinese populations. This study implies that np 16129 can be used as a marker for Malaysian population. For further investigation, the length of the target sequence may be increased to obtain more variations that can be used as markers. This will increase the discrimination power of Malaysian population.

Original languageEnglish
Pages (from-to)843-847
Number of pages5
JournalMitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
Volume30
Issue number8
DOIs
Publication statusPublished - 17 Nov 2019

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Mitochondrial DNA
Population
DNA
Cheek
Nucleotides
Polymerase Chain Reaction

Keywords

  • DNA variations
  • haplogroup
  • human population
  • mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Variations in mitochondrial DNA control region among Malay and Chinese subpopulations (sequence 16000–16200). / Ishar, Seri Mirianti; Parameswaran, Kiroobhashini; Masduki, Nur Safrina; Rus Din, Rus Dina.

In: Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, Vol. 30, No. 8, 17.11.2019, p. 843-847.

Research output: Contribution to journalArticle

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