Pengesahan bagi mengesan bakteria patogen makanan menggunakan teknologi mikroaturan DNA

Translated title of the contribution: Validation of food-borne pathogen detection using DNA microarray technology

Research output: Contribution to journalArticle

Abstract

This study was conducted to validate the capability of DNA microarray technology of OliproTM FoodPATH Gene Chip in detecting food-borne pathogens. Nine types of food-borne pathogen DNAs were used namely Bacillus cereus, Escherichia coli 0157:H7, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella spp., Shigella spp. and Campylobacter spp. A total of 36 combinations of DNA templates of food-borne pathogens were used. Polymerase chain reaction (PCR) and Southern-Blotting Hybridization methods were used on the gene chip to validate its capability. The results of hibridization on gene chip were compared with results from gel electrophoresis of 2.0% (w/v). Five screenings were required to complete the 36 combinations of DNA templates of food-borne pathogen. For each screening, one gene chip was used as negative control, whereby no DNA templates of food-borne pathogen were inoculated. The results showed that all combinations of food-borne pathogen DNA templates were detected. The negative control chip did not show the presence of any DNA. This study showed that OliproTM FoodPATH Gene Chip gave better results than the 2.0% (w/v) gel electrophoresis.

Original languageUndefined/Unknown
Pages (from-to)1715-1720
Number of pages6
JournalSains Malaysiana
Volume42
Issue number12
Publication statusPublished - Dec 2013

Fingerprint

microbial detection
microarray technology
food pathogens
DNA
gel electrophoresis
screening
Vibrio cholerae
Shigella
Vibrio parahaemolyticus
Campylobacter
Bacillus cereus
Southern blotting
Listeria monocytogenes
DNA microarrays
Staphylococcus aureus
Salmonella
hybridization
polymerase chain reaction
Escherichia coli

Keywords

  • DNA microarray
  • Food-borne pathogen
  • OliproTM FoodPATH gene ghip

ASJC Scopus subject areas

  • General

Cite this

Pengesahan bagi mengesan bakteria patogen makanan menggunakan teknologi mikroaturan DNA. / Sawei, Jelin; Abdullah Sani, Norrakiah; Abdullah, Aminah; Abd. Mutalib, Sahilah.

In: Sains Malaysiana, Vol. 42, No. 12, 12.2013, p. 1715-1720.

Research output: Contribution to journalArticle

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