Utilization of STMS markers to verify admixture in clonal progenies of Acacia mapping populations and relabelling using assignment tests

M. J. Asif, M. A T Ariffin, H. M. Yit, M. Wong, M. Z. Abdullah, N. Muhammad, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Clonal propagation is widely used for Acacia breeding and commercial planting. When a large number of clones are handled, problems with mixings are commonly confronted. Detection of admixture in Acacia clones based on morphology particularly at seedling stage is not feasible. However, molecular markers are commonly used to test the genetic fidelity of planting materials. This paper reports the detection of mislabelling in Acacia clonal progenies using a sequence tagged microsatellite (STMS) genetic marker system. Progenies from two mapping populations were clonally propagated and field planted for phenotypic and genotypic evaluation at three locations in Malaysia: (a) Forest Research Institute Malaysia field station at Segamat, Johor, (b) Borneo Tree Seeds and Seedlings Supplies Sdn, Bhd. (BTS) field trial site at Bintulu, Sarawak and (c) Asiaprima RCF field trial site at Lancang, Pahang. During field planting mislabelling was reported at Segamat, Johor and similar was suspected for Bintulu, Sarawak. Screening revealed mislabelling events in both populations. A total of 18.52% mislabelling incidences were detected from both sites, of which 17.39% of mislabelling was detected for fibre length cross and 20% for wood density cross. The assignment test efficiently reestablished the mislabelled ramets to the respective clones. Future studies should be focused on the utilization of a higher number of markers, e.g. SSR or SNPs to increase a discrimination power. A high number of SNPs can be generated within a short period of time compared to SSR, but SNPs could be cost inhibitory. Multiplexing microsatellite combinations along with sample bulking will further reduce the processing time when screening large populations. The use of assignment test would efficiently assign mislabelled individuals to the respective clones. It is concluded that checking for mislabelling is imperative for future breeding and for analyses such as QTL mapping where a correlation between genotypic and phenotypic data is determined.

Original languageEnglish
Pages (from-to)200-209
Number of pages10
JournalJournal of Forest Science
Volume61
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Acacia
clone
Borneo
microsatellite repeats
clones
planting
Malaysia
field experimentation
testing
breeding
seedling
screening
genetic markers
seed trees
seedlings
wood density
genetic marker
quantitative trait loci
seed
incidence

Keywords

  • Dna markers
  • Linkage mapping
  • Quantitative trait loci (qtl)
  • Tree breeding

ASJC Scopus subject areas

  • Forestry
  • Soil Science

Cite this

Utilization of STMS markers to verify admixture in clonal progenies of Acacia mapping populations and relabelling using assignment tests. / Asif, M. J.; Ariffin, M. A T; Yit, H. M.; Wong, M.; Abdullah, M. Z.; Muhammad, N.; V Ratnam, R Wickneswari.

In: Journal of Forest Science, Vol. 61, No. 5, 2015, p. 200-209.

Research output: Contribution to journalArticle

Asif, M. J. ; Ariffin, M. A T ; Yit, H. M. ; Wong, M. ; Abdullah, M. Z. ; Muhammad, N. ; V Ratnam, R Wickneswari. / Utilization of STMS markers to verify admixture in clonal progenies of Acacia mapping populations and relabelling using assignment tests. In: Journal of Forest Science. 2015 ; Vol. 61, No. 5. pp. 200-209.
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