Utilization of palm kernel cake for production of β-glucosidase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor

P. Abdeshahian, N. Samat, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The production of β-glucosidase by Aspergillus niger FTCC 5003 from palm kernel cake as a substrate was studied in solid substrate fermentation using a laboratory column bioreactor. The simultaneous effects of three independent variables, namely incubation temperature, initial moisture content of substrate and airflow rate on the production of β-glucosidase were evaluated using response surface methodology on the basis of a central composite face-centered design. A total of 18 experiments were carried out in which Aspergillus niger FTCC 5003 was cultivated on PKC in an aerated column bioreactor for 7 days under incubation temperature, moisture level and aeration rate determined. Experimental results showed that the highest activity of β-glucosidase (52.06 U g-1) was obtained at an incubation temperature, an initial moisture level and an aeration rate of 32.5°C, 60% and 1.5 L min-1, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had highly significant effects on the production of β-glucosidase (p<0.01). The statistical results also showed that the interaction effect between initial moisture level and aeration rate significantly influenced the production of β-glucosidase (p<0.05). Optimum conditions suggested by the second-order polynomial regression model for attaining maximum β-glucosidase production were 31.6°C incubation temperature, 57.0% initial moisture and 0.5 L min-1 aeration rate with a predicted production value of 47.20 Ug-1.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalBiotechnology
Volume9
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Glucosidases
Aspergillus niger
Aspergillus
Bioreactors
Fermentation
Moisture
Substrates
Temperature
Statistical Models
Statistical methods
Polynomials
Composite materials

Keywords

  • β-glucosidase
  • Aspergillus niger
  • Column bioreactor
  • Palm kernel cake
  • Solid substrate fermentation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Utilization of palm kernel cake for production of β-glucosidase by Aspergillus niger FTCC 5003 in solid substrate fermentation using an aerated column bioreactor. / Abdeshahian, P.; Samat, N.; Wan Yusoff, Wan Mohtar.

In: Biotechnology, Vol. 9, No. 1, 2010, p. 17-24.

Research output: Contribution to journalArticle

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