Utilization of palm kernel cake as a renewable feedstock for fermentative hydrogen production

Nadia Farhana Azman, Peyman Abdeshahian, Abudukeremu Kadier, Hafiza Shukor, Najeeb Kaid Nasser Al-Shorgani, Aidil Abdul Hamid, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fermentative hydrogen generation was studied using palm kernel cake (PKC) as sustainable cellulosic biomass. PKC was subjected to an acid hydrolysis approach using dilute H2SO4 (7% v/v). PKC hydrolysate obtained was then diluted (70%) and used as a substrate for hydrogen generation. Chemical analysis showed that the main fermentable sugars in diluted PKC hydrolysate were glucose, xylose and mannose with the concentrations of 2.75 g/L, 2.60 g/L and 27.75 g/L, respectively. Hydrogen production was carried out by the cultivation of Clostridium acetobutylicum YM1 on PKC hydrolysate. The effect of incubation temperature, the initial pH of culture medium and microbial inoculum size on hydrogen production was studied using a statistical model. The analysis of the model generated showed that the initial pH value of the culture medium and inoculum size had significant effects on the hydrogen production. The study showed that the optimum conditions for the biohydrogen production were 30.57 °C temperature, pH 5.5 and 20% inoculum size. A verification experiment was performed in the optimum conditions determined. Experimental results of the verification test showed that a cumulative hydrogen volume of 1575 ml/L was generated with consuming 2.75 g/L glucose, 2.20 g/L xylose and 16.31 g/L mannose.

Original languageEnglish
Pages (from-to)700-708
Number of pages9
JournalRenewable Energy
Volume93
DOIs
Publication statusPublished - 1 Aug 2016

Fingerprint

Hydrogen production
Feedstocks
Xylose
Hydrogen
Glucose
Clostridium
Sugars
Hydrolysis
Biomass
Temperature
Acids
Substrates
Chemical analysis
Experiments

Keywords

  • Acid hydrolysis
  • Clostridium acetobutylicum YM1
  • Fermentable sugar
  • Hydrogen production
  • Optimisation
  • Palm kernel cake

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

Cite this

Utilization of palm kernel cake as a renewable feedstock for fermentative hydrogen production. / Azman, Nadia Farhana; Abdeshahian, Peyman; Kadier, Abudukeremu; Shukor, Hafiza; Al-Shorgani, Najeeb Kaid Nasser; Abdul Hamid, Aidil; Kalil, Mohd. Sahaid.

In: Renewable Energy, Vol. 93, 01.08.2016, p. 700-708.

Research output: Contribution to journalArticle

Azman, Nadia Farhana ; Abdeshahian, Peyman ; Kadier, Abudukeremu ; Shukor, Hafiza ; Al-Shorgani, Najeeb Kaid Nasser ; Abdul Hamid, Aidil ; Kalil, Mohd. Sahaid. / Utilization of palm kernel cake as a renewable feedstock for fermentative hydrogen production. In: Renewable Energy. 2016 ; Vol. 93. pp. 700-708.
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