Using wintergreen oil for mounting mosquito larvae: A safer alternative to xylene

J. B. Koay, N. N. Natasya, M. A G Nashithatul, R. Ihsanuddin, F. M. Salleh, Aishah Hani Azil

Research output: Contribution to journalArticle

Abstract

Permanent mounting of fourth instar mosquito larvae is essential for identifying Aedes spp. This procedure requires extensive exposure to xylene, a clearing agent in the mounting process. We investigated wintergreen oil as a substitute for xylene. Five hundred larvae were mounted on slides to evaluate shrinkage or expansion of specimens after clearing using xylene or wintergreen oil. We examined the ventral brush and siphonal hair tufts for species identification and for preservation of morphological characteristics after clearing specimens in xylene or wintergreen oil. Shrinkage of the length of whole larvae and width of the head, thorax and abdomen after mounting was significantly greater after clearing with xylene than with wintergreen oil. The length of the comb scale nearest the ventral brush was similar for both clearing agents. The clarity of the specimens after mounting was improved by clearing with wintergreen oil, but the integrity of the ventral brush and siphonal hair tufts were similar for both clearing agents.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBiotechnic and Histochemistry
Volume91
Issue number1
DOIs
Publication statusPublished - 2 Jan 2016

Fingerprint

Xylenes
Culicidae
Larva
Comb and Wattles
Aedes
Abdomen
Thorax
Head
wintergreen oil

Keywords

  • Aedes
  • clearing
  • dengue
  • laboratory technicians
  • larvae
  • methyl salicylate
  • mosquito
  • mounting
  • occupational hazard
  • shrinkage
  • wintergreen oil
  • xylene

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Cite this

Using wintergreen oil for mounting mosquito larvae : A safer alternative to xylene. / Koay, J. B.; Natasya, N. N.; Nashithatul, M. A G; Ihsanuddin, R.; Salleh, F. M.; Azil, Aishah Hani.

In: Biotechnic and Histochemistry, Vol. 91, No. 1, 02.01.2016, p. 63-70.

Research output: Contribution to journalArticle

Koay, J. B. ; Natasya, N. N. ; Nashithatul, M. A G ; Ihsanuddin, R. ; Salleh, F. M. ; Azil, Aishah Hani. / Using wintergreen oil for mounting mosquito larvae : A safer alternative to xylene. In: Biotechnic and Histochemistry. 2016 ; Vol. 91, No. 1. pp. 63-70.
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