Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs

Son Le, Christopher E. Harwood, A. Rod Griffin, Son H. Do, Thinh H. Ha, R Wickneswari V Ratnam, René E. Vaillancourt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We used a set of 16 SSR markers to check the identity of pure-species and hybrid clones in Vietnam’s Acacia auriculiformis, Acacia mangium, and acacia hybrid (A. mangium × A. auriculiformis) breeding programs. The statistics package HIest, applied to a large synthesized population, enabled accurate allocation of genotypes to the two pure species, F1 and F2 inter-specific hybrids and backcrosses, based on estimates of hybridity and heterozygosity. The hybridity status of putatively pure A. mangium and A. auriculiformis clones in adjacent clonal seed orchards was checked. Four out of 100 clones selected as A. mangium were found to be backcrosses (A. mangium × F1 inter-specific hybrid) while out of 96 clones selected as A. auriculiformis, two were F1 hybrids and two were backcrosses (A. auriculiformis × F1 hybrid). The markers were then applied to check the hybridity status of 160 putative acacia F1 hybrid genotypes that had been selected on morphological criteria from open-pollinated progenies collected from A. auriculiformis and A. mangium parents. Many selections based on morphology were found to be mistaken. Only thirteen of 63 clones originating from A. auriculiformis mothers were F1 hybrids, four were backcrosses, and the remaining 46 were pure A. auriculiformis. Fewer mistakes were evident for clones selected from A. mangium mothers, with 82 out of 89 clones confirmed as F1 hybrids, three as backcrosses, and four as pure A. mangium. The occurrence of F1 hybrids and backcrosses in pure-species seed orchards and their progeny shows that inter-species contamination is an issue requiring management in both pure-species and in hybrid breeding of these species in Vietnam. Examination of genetic distances among verified clones showed patterns of relatedness that were consistent with pedigree records. Implications for resource management as well as for breeding and clonal selection strategies are considered.

Original languageEnglish
Article number102
JournalTree Genetics and Genomes
Volume13
Issue number5
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

Acacia
natural resource management
Acacia auriculiformis
Acacia mangium
Breeding
resource management
Clone Cells
breeding
clone
clones
seed orchards
Seeds
Genotype
orchard
genotype
programme
marker
Vietnam
Pedigree
seed

Keywords

  • A. mangium
  • Acacia auriculiformis
  • Acacia hybrid
  • Hybrid detection
  • Hybridity
  • SSR markers

ASJC Scopus subject areas

  • Forestry
  • Molecular Biology
  • Genetics
  • Horticulture

Cite this

Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs. / Le, Son; Harwood, Christopher E.; Rod Griffin, A.; Do, Son H.; Ha, Thinh H.; V Ratnam, R Wickneswari; Vaillancourt, René E.

In: Tree Genetics and Genomes, Vol. 13, No. 5, 102, 01.10.2017.

Research output: Contribution to journalArticle

Le, Son ; Harwood, Christopher E. ; Rod Griffin, A. ; Do, Son H. ; Ha, Thinh H. ; V Ratnam, R Wickneswari ; Vaillancourt, René E. / Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs. In: Tree Genetics and Genomes. 2017 ; Vol. 13, No. 5.
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