Use of Fe3O4 nanoparticles for enhancement of biosensor response to the herbicide 2,4-dichlorophenoxyacetic acid

Kee Shyuan Loh, Yook Heng Lee, Musa Ahmad, Abdul Aziz Salmah, Ishak Zamri

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Magnetic nanoparticles of Fe3O4 were synthesized and characterized using transmission electron microscopy and X-ray diffraction. The Fe3O4 nanoparticles were found to have an average diameter of 5.48 ±1.37 nm. An electrochemical biosensor based on immobilized alkaline phosphatase (ALP) and Fe3O4 nanoparticles was studied. The amperometric biosensor was based on the reaction of ALP with the substrate ascorbic acid 2-phosphate (AA2P). The incorporation of the Fe 3O4 nanoparticles together with ALP into a sol gel/chitosan biosensor membrane has led to the enhancement of the biosensor response, with an improved linear response range to the substrate AA2P (5-120 μM) and increased sensitivity. Using the inhibition property of the ALP, the biosensor was applied to the determination of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). The use of Fe3O4 nanoparticles gives a two-fold improvement in the sensitivity towards 2,4-D, with a linear response range of 0.5-30 μgL-1 Exposure of the biosensor to other toxicants such as heavy metals demonstrated only slight interference from metals such as Hg2+, Cu2+, Ag 2+ and Pb2+. The biosensor was shown to be useful for the determination of the herbicide 2, 4-D because good recovery of 95-100 percent was obtained, even though the analysis was performed in water samples with a complex. matrix. Furthermore, the results from the analysis of 2,4-D in water samples using the biosensor correlated well with a HPLC method.

Original languageEnglish
Pages (from-to)5775-5791
Number of pages17
JournalSensors
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

herbicides
2,4-Dichlorophenoxyacetic Acid
Herbicides
Biosensing Techniques
bioinstrumentation
Biosensors
Nanoparticles
nanoparticles
acids
phosphatases
Acids
augmentation
Phosphatases
Alkaline Phosphatase
ascorbic acid
Ascorbic acid
phosphates
Phosphates
Enzyme inhibition
Water

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Alkaline phosphatase
  • FeO nanoparticles
  • Screen-printer biosensor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

Cite this

Use of Fe3O4 nanoparticles for enhancement of biosensor response to the herbicide 2,4-dichlorophenoxyacetic acid. / Loh, Kee Shyuan; Lee, Yook Heng; Ahmad, Musa; Salmah, Abdul Aziz; Zamri, Ishak.

In: Sensors, Vol. 8, No. 9, 09.2008, p. 5775-5791.

Research output: Contribution to journalArticle

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