Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612

In Seop Chang, Daehee Kim, Byung Hong Kim, Robert W. Lovitt

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Studies were made on the composition of the growth medium to increase the cell concentration in a cell-recycled continuous culture (Eubacterium limosum KIST612) with carbon monoxide as a sole energy source using phosphate-buffered basal medium (PBBM) and modified PBBM. One of major limiting factors in PBBM might be nitrogen during the high cell density culture. This limitation could be overcome by increasing of inorganic nitrogen or yeast extract concentration in the medium. Anaerobic digester fluid, which could replace the organic nitrogen in PBBM, was used to develop an industrial grade medium for conversion of CO to multi-carbon compound.

Original languageEnglish
Pages (from-to)1183-1187
Number of pages5
JournalBiotechnology Letters
Volume29
Issue number8
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

Fingerprint

Eubacterium
Carbon Monoxide
Fermentation
Phosphates
Cell Count
Nitrogen
Continuous cell culture
Carbon monoxide
Yeast
Carbon
Cell Culture Techniques
Yeasts
Fluids
Growth
Chemical analysis

Keywords

  • Carbon monoxide
  • Eubacterium limosum
  • Industrial grade medium

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612. / Chang, In Seop; Kim, Daehee; Kim, Byung Hong; Lovitt, Robert W.

In: Biotechnology Letters, Vol. 29, No. 8, 08.2007, p. 1183-1187.

Research output: Contribution to journalArticle

Chang, In Seop ; Kim, Daehee ; Kim, Byung Hong ; Lovitt, Robert W. / Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612. In: Biotechnology Letters. 2007 ; Vol. 29, No. 8. pp. 1183-1187.
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