Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses

Jiyoung Lee, Nguyet Thu Phung, In Seop Chang, Byung Hong Kim, Ha Chin Sung

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

A fuel cell-type electrochemical device has been used to enrich microbes oxidizing acetate with concomitant electricity generation without using an electron mediator from activated sludge. The device generated a stable current of around 5 mA with complete oxidation of 5 mM acetate at the hydraulic retention time of 2.5 h after 4 weeks of enrichment. Over 70% of electrons available from acetate oxidation was recovered as current. Carbon monoxide or hydrogen did not influence acetate oxidation or current generation from the microbial fuel cell (MFC). Denaturing gradient gel electrophoresis showed that DNA extracted from the acetate-enriched MFC had different 16S rDNA patterns from those of sludge or glucose+glutamate-enriched MFCs. Nearly complete 16S rDNA sequence analyses showed that diverse bacteria were enriched in the MFC fed with acetate. Electron microscopic observations showed biofilm developed on the electrode, but not microbial clumps observed in MFCs fed with complex fuel such as glucose and wastewater from a corn-processing factory.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalFEMS Microbiology Letters
Volume223
Issue number2
DOIs
Publication statusPublished - 27 Jun 2003
Externally publishedYes

Fingerprint

Ribosomal DNA
Acetates
Bioelectric Energy Sources
Electrons
Sewage
Glucose
Equipment and Supplies
Electricity
Denaturing Gradient Gel Electrophoresis
Carbon Monoxide
Biofilms
Waste Water
Zea mays
Sequence Analysis
Glutamic Acid
Hydrogen
Electrodes
Bacteria
DNA

Keywords

  • Acetate oxidation
  • Anaerobic respiration
  • Electrochemically active bacterium
  • Mediator-less microbial fuel cell

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses. / Lee, Jiyoung; Phung, Nguyet Thu; Chang, In Seop; Kim, Byung Hong; Sung, Ha Chin.

In: FEMS Microbiology Letters, Vol. 223, No. 2, 27.06.2003, p. 185-191.

Research output: Contribution to journalArticle

Lee, Jiyoung ; Phung, Nguyet Thu ; Chang, In Seop ; Kim, Byung Hong ; Sung, Ha Chin. / Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses. In: FEMS Microbiology Letters. 2003 ; Vol. 223, No. 2. pp. 185-191.
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