Uptake of triphenylmethane and cellular localization of enzyme for its decolorization in enterobacter cloacae MG82

Min Seon Jeong, Soon Jun Kwak, Byung Hong Kim, Young Gun Chung, Sa Ouk Kang, Kyung Hee Min

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Triphenylmethane was decolorized rapidly by Enterbacter cloacae MG 82 at initial reaction time. The spheroplast showed higher activity of tripheriylmethane decolorization than that of intact cell suspension. The outer part of the bacterial cell envelope and the peptidoglycan are important for the function of transport barrier of triphenylmethane. In intact cell, decolorization activity was higher at 37°c than at 0°C, indicating that triphenylmethane decolorization is due to the enzyme reaction. Culture filtrate showed no decolorization activity, while cell-free extract appeared high activity of 1.45 units, clearly showing that decolorization activity was due to the cell-free extract. Comparing decolorization activities of cell fractions, it was found that decolorization activity was located at the compartment of cytoplasmic membrane. The enzyme activity was also shown to be Mg++-dependent. The optimum pH and temperature of enzyme activity were 7.0 and 50°C, respectively. The thermostability of this enzyme at 35°C was kept to 58% for 3 hours.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalKorean Journal of Applied Microbiology and Biotechnology
Volume25
Issue number1
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Enterobacter cloacae
Enzymes
Cell Extracts
Spheroplasts
Cloaca
Peptidoglycan
Suspensions
Cell Membrane
Temperature
triphenylmethane

Keywords

  • Cytoplasmic membrane bound
  • Decolonization activity
  • Mg-dependent enzyme
  • Triphenylmethane dye
  • Uptake

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Uptake of triphenylmethane and cellular localization of enzyme for its decolorization in enterobacter cloacae MG82. / Jeong, Min Seon; Kwak, Soon Jun; Kim, Byung Hong; Chung, Young Gun; Kang, Sa Ouk; Min, Kyung Hee.

In: Korean Journal of Applied Microbiology and Biotechnology, Vol. 25, No. 1, 1997, p. 37-43.

Research output: Contribution to journalArticle

Jeong, Min Seon ; Kwak, Soon Jun ; Kim, Byung Hong ; Chung, Young Gun ; Kang, Sa Ouk ; Min, Kyung Hee. / Uptake of triphenylmethane and cellular localization of enzyme for its decolorization in enterobacter cloacae MG82. In: Korean Journal of Applied Microbiology and Biotechnology. 1997 ; Vol. 25, No. 1. pp. 37-43.
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AU - Min, Kyung Hee

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