Upregulation of SOX-2, FZD9, Nestin, OCT-4 and FGF-4 expression in human chorion derived-stem cells after angiogenic induction.

H. Abdul Rahman, N. F Mohd Manzor, Geok Chin Tan, A. E. Tan, Chua Kien Hui

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Angiogenic induction was made to promote angiogenesis by differentiating stem cells towards endothelial cells. However, the stemness property of induced cells has not been revealed yet. Hence, we aim to evaluate the differential mRNA expression of stemness genes in human chorion-derived stem cells (CDSC) after being cultured in EDM50 comprised bFGF and VEGF. Results indicated that CDSC cultured in EMD50 expressed significantly higher mRNA level of Sox-2, FZD9, BST-1 and Nestin. In addition Oct-4, FGF-4 and ABCG-2 were also upregulated. Our finding suggested that CDSC after angiogenic induction enhanced its stem cell properties. This could be contributed for the mechanism of stem cell therapy in ischemic problem.

Original languageEnglish
Pages (from-to)57-58
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Chorion
Nestin
Up-Regulation
Stem Cells
Messenger RNA
Cell- and Tissue-Based Therapy
Vascular Endothelial Growth Factor A
Endothelial Cells
Gene Expression

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Upregulation of SOX-2, FZD9, Nestin, OCT-4 and FGF-4 expression in human chorion derived-stem cells after angiogenic induction. / Abdul Rahman, H.; Manzor, N. F Mohd; Tan, Geok Chin; Tan, A. E.; Kien Hui, Chua.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 57-58.

Research output: Contribution to journalArticle

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abstract = "Angiogenic induction was made to promote angiogenesis by differentiating stem cells towards endothelial cells. However, the stemness property of induced cells has not been revealed yet. Hence, we aim to evaluate the differential mRNA expression of stemness genes in human chorion-derived stem cells (CDSC) after being cultured in EDM50 comprised bFGF and VEGF. Results indicated that CDSC cultured in EMD50 expressed significantly higher mRNA level of Sox-2, FZD9, BST-1 and Nestin. In addition Oct-4, FGF-4 and ABCG-2 were also upregulated. Our finding suggested that CDSC after angiogenic induction enhanced its stem cell properties. This could be contributed for the mechanism of stem cell therapy in ischemic problem.",
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AU - Tan, A. E.

AU - Kien Hui, Chua

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