Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice

Izwan Bharudin, Mohd Faizal Abu Bakar, Noor Haza Fazlin Hashim, Mohd Noor Mat Isa, Halimah Alias, Mohd Firdaus Mohd Raih, Rosli Md Illias, Nazalan Najimudin, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and −12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each library were randomly picked and sequenced. Comparative analyses from the resulting ESTs in each condition identified several groups of genes required for cold adaptation. Additionally, 319 unique transcripts that encoded uncharacterised functions were identified in the −12 °C library and are currently unique to G. antarctica. Gene expression analysis using RT-qPCR revealed two of the unknown genes to be up-regulated at −12 °C compared to 0 °C and 15 °C. These findings further contribute to the collective knowledge into G. antarctica cold adaptation and as a resource for understanding the ecological and physiological tolerance of psychrophilic microbes in general.

Original languageEnglish
JournalMarine Environmental Research
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Sea ice
Antarctica
sea ice
ice
Genes
gene
genes
expressed sequence tags
Gene expression
Yeast
gene expression
yeast
clone
tolerance
clones
yeasts
microorganisms
resource
cold
temperature

Keywords

  • Antarctica
  • Antifreeze proteins
  • Cold adaptation
  • EST
  • Glaciozyma antarctica
  • Lipid content
  • mRNA
  • Psychrophilic
  • Sea ice

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science
  • Pollution

Cite this

Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice. / Bharudin, Izwan; Abu Bakar, Mohd Faizal; Hashim, Noor Haza Fazlin; Mat Isa, Mohd Noor; Alias, Halimah; Mohd Raih, Mohd Firdaus; Md Illias, Rosli; Najimudin, Nazalan; Mahadi, Nor Muhammad; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir.

In: Marine Environmental Research, 01.01.2018.

Research output: Contribution to journalArticle

Bharudin, Izwan ; Abu Bakar, Mohd Faizal ; Hashim, Noor Haza Fazlin ; Mat Isa, Mohd Noor ; Alias, Halimah ; Mohd Raih, Mohd Firdaus ; Md Illias, Rosli ; Najimudin, Nazalan ; Mahadi, Nor Muhammad ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir. / Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice. In: Marine Environmental Research. 2018.
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