Unlocking “silent” genes via combine culture–an alternative gateway to natural products discovery

Izzatul Hidayah Azizan, Jalifah Latip, Shotaro Hoshino, Ikuro Abe

Research output: Contribution to journalArticle

Abstract

Streptomyces sp. has been known to produce antibiotics and other bioactive natural products. However, the production of these secondary metabolites depends on the culture conditions, where in most cases the secondary-metabolite genes are not expressed in fermentation culture. Recently, a novel fermentation method known as combined-culture has been introduced to unlock these “silent” genes, hence induces the production of cryptic metabolites. We report herein, our preliminary work on combined-culture using two soil-borne bacterial strains; Streptomyces and Tsukamurella. From the results, it is shown that the presence of Tsukamurella, a mycolic acid-containing bacterium induces the production of new metabolites in Streptomyces. Moreover, the production of compounds associated with Streptomyces was enhanced via combination-culture as compared to culture of Streptomyces strain alone. These findings may promote the feasibility of combined-culture in unlocking the “silent” genes of microorganisms which could lead to the discovery of novel metabolites.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalJurnal Teknologi
Volume77
Issue number2
Publication statusPublished - 2015

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Metabolites
Genes
Cell culture
Fermentation
Antibiotics
Microorganisms
Bacteria
Soils
Acids

Keywords

  • Combined culture
  • Streptomyces
  • Tsukamurella

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Unlocking “silent” genes via combine culture–an alternative gateway to natural products discovery. / Azizan, Izzatul Hidayah; Latip, Jalifah; Hoshino, Shotaro; Abe, Ikuro.

In: Jurnal Teknologi, Vol. 77, No. 2, 2015, p. 7-11.

Research output: Contribution to journalArticle

Azizan, Izzatul Hidayah ; Latip, Jalifah ; Hoshino, Shotaro ; Abe, Ikuro. / Unlocking “silent” genes via combine culture–an alternative gateway to natural products discovery. In: Jurnal Teknologi. 2015 ; Vol. 77, No. 2. pp. 7-11.
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