Ultrastructural studies of fresh, frozen-thawed and acrosome-reacted goat sperm

N. H. Hashida, R. B. Abdullah, M. H. Rajikin, Mahanem Mat Noor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Data on the ultrastructure of sperm subjected to freezing and acrosome reaction treatment are lacking. The present study was undertaken to investigate the effects of sperm freezing and acrosome reaction on the ultrastructure of the goat sperm. The sperm were subjected to slow freezing method using programmable freezer at 4°C for 3.5 hours and acrosome reaction treatment prior to Transmission Electron Microscopy (TEM) procedures. The results showed that the plasma and acrosomal membranes of the frozen-thawed sperm were expanded and the mitochondria became irregular in shape. On the otherhand, acrosome-reacted sperm showed ruptured plasma and acrosomal membrane and dispersion of the acrosome matrix. Frozen-thawed sperm also showed the dispersion of the acrosomal matrix within the plasma membrane. The nine outer-dense fibres of frozen-thawed and acrosome-reacted sperms were irregular in shape. No noticeable difference in the axoneme complex arrangement was observed in both samples. It can be concluded from our findings that the noticeable difference of the membranes and the mitochondria may cause the decrease in the sperm motility and subsequently a decrease in the rate of in vitro fertilization.

Original languageEnglish
Pages (from-to)119-123
Number of pages5
JournalBiomedical Research
Volume16
Issue number2
Publication statusPublished - May 2005

Fingerprint

Acrosome
Goats
Freezing
Spermatozoa
Mitochondria
Membranes
Acrosome Reaction
Plasmas
Cell membranes
Cell Membrane
Transmission electron microscopy
Fibers
Axoneme
Sperm Motility
Fertilization in Vitro
Transmission Electron Microscopy
Therapeutics

Keywords

  • Acrosome-reacted sperm
  • Fresh sperm
  • Frozen-thawed
  • Goat sperm
  • Ultrastructure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hashida, N. H., Abdullah, R. B., Rajikin, M. H., & Mat Noor, M. (2005). Ultrastructural studies of fresh, frozen-thawed and acrosome-reacted goat sperm. Biomedical Research, 16(2), 119-123.

Ultrastructural studies of fresh, frozen-thawed and acrosome-reacted goat sperm. / Hashida, N. H.; Abdullah, R. B.; Rajikin, M. H.; Mat Noor, Mahanem.

In: Biomedical Research, Vol. 16, No. 2, 05.2005, p. 119-123.

Research output: Contribution to journalArticle

Hashida, NH, Abdullah, RB, Rajikin, MH & Mat Noor, M 2005, 'Ultrastructural studies of fresh, frozen-thawed and acrosome-reacted goat sperm', Biomedical Research, vol. 16, no. 2, pp. 119-123.
Hashida, N. H. ; Abdullah, R. B. ; Rajikin, M. H. ; Mat Noor, Mahanem. / Ultrastructural studies of fresh, frozen-thawed and acrosome-reacted goat sperm. In: Biomedical Research. 2005 ; Vol. 16, No. 2. pp. 119-123.
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