ULTRASTRUCTURAL RESPONSE OF EMBRYONIC AXES OF Fortunella polyandra TO DEHYDRATION AND CRYOPRESERVATION

Omar M. Al Zoubi, Normah Mohd. Noor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BACKGROUND: To further understand the survival characteristics of desiccation-sensitive excised embryonic axes of Fortunella polyandra to desiccation and cryopreservation it is necessary to study the impact of drying rates on both the ultrastructure and electrolyte leakage.

OBJECTIVE: To examine the effects of two different drying regimes (silica gel and ultra-rapid) on the survival, ultrastructure and membrane leakage characteristics of excised embryonic axes of F. polyandra before and after cryopreservation.

MATERIALS AND METHODS: The effects of the drying regimes on the survival, ultrastructure and membrane integrity of the excised embryonic axes of F. polyandra was determined. Survival was assessed in vitro, and the integrity of membranes following drying was estimated by electrolyte leakage and observation under the transmission electron microscope (TEM). Survival and ultrastructural changes were also observed after cryopreservation.

RESULTS: Electrolyte leakage increased with decreasing water content of the embryonic axes, indicative of substantial subcellular damage, after both ultra-rapid dehydration (to water contents of

CONCLUSION: For surviving axes, the damage was less severe and the axes grew to become normal seedlings. Ultrastructural studies reveal the damage of the cells at different rates of dehydration and during cryopreservation.

Original languageEnglish
Pages (from-to)379-391
Number of pages13
JournalCryo-Letters
Volume36
Issue number6
Publication statusPublished - 1 Nov 2015

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Fortunella
Rutaceae
Cryopreservation
Dehydration
cryopreservation
drying
Electrolytes
electrolytes
Desiccation
ultrastructure
Membranes
water content
transmission electron microscopes
Water
Silica Gel
silica gel
Seedlings
Observation
Electrons
seedlings

ASJC Scopus subject areas

  • Medicine(all)

Cite this

ULTRASTRUCTURAL RESPONSE OF EMBRYONIC AXES OF Fortunella polyandra TO DEHYDRATION AND CRYOPRESERVATION. / Al Zoubi, Omar M.; Mohd. Noor, Normah.

In: Cryo-Letters, Vol. 36, No. 6, 01.11.2015, p. 379-391.

Research output: Contribution to journalArticle

Al Zoubi, Omar M. ; Mohd. Noor, Normah. / ULTRASTRUCTURAL RESPONSE OF EMBRYONIC AXES OF Fortunella polyandra TO DEHYDRATION AND CRYOPRESERVATION. In: Cryo-Letters. 2015 ; Vol. 36, No. 6. pp. 379-391.
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