Ultrastructural analysis of Apicomplexa-Like parasites in two conch species Laevistrombus canarium and canarium urceus from Johor Straits, Malaysia

Nur Fauzana Azmi, Mazlan Abd. Ghaffar, Hassan Hj Mohd Daud, Zaidi Che Cob

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The tropical conch, Laevistrombus canarium (Linnaeus, 1758) and Canarium urceus (Linneaus, 1758) are ecologically and economically important shellfish species in Malaysia and neighboring region. Their populations, however are currently declining and this histopathological study investigates the aspect of parasitism and diseases that may affect their well-being. Conch samples were randomly collected from their natural habitat and histological sections (4–5 µm) of various organs and tissues were examined under light microscope. This was followed by ultrastructure analysis on infected tissues using transmission electron microscope (TEM). Based on the histological analysis, large numbers of gamonts, sporocysts and trophozoites of Apicomplexa-like parasites were observed in the vacuolated cells and pyramidal crypt cells of the digestive tubules, and in the digestive ducts. Furthermore, coccidian and oocysts-like Pseudoklossia sp. stages were also observed in the cells of the kidney. Apart from that, spores with cyst-like structure were observed in the digestive gland and kidney. Although the parasites were present in most of the organs analyzed, there was no obvious symptom, inflammatory response or mortality incurred on both species, which implies the possibility of a non-virulent relationship like commensalisms or mutualism. However, more investigations, including molecular studies, are needed to confirm the parasite identification and dynamics, and to further evaluate the nature of relationship between Apicomplexa parasites and their host.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalJournal of Invertebrate Pathology
Volume152
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Canarium
Malaysia
strait
parasite
parasites
gametocytes
trophozoites
sporocysts (Protozoa)
transmission electron microscopes
mutualism
light microscopes
ultrastructure
kidney cells
shellfish
cyst
parasitism
oocysts
signs and symptoms (animals and humans)
spore
spores

Keywords

  • Apicomplexa
  • Canarium urceus
  • Coccidia
  • Laevistrombus canarium

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Ultrastructural analysis of Apicomplexa-Like parasites in two conch species Laevistrombus canarium and canarium urceus from Johor Straits, Malaysia. / Azmi, Nur Fauzana; Abd. Ghaffar, Mazlan; Daud, Hassan Hj Mohd; Che Cob, Zaidi.

In: Journal of Invertebrate Pathology, Vol. 152, 01.02.2018, p. 17-24.

Research output: Contribution to journalArticle

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