Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Mahbubur Rahman, Yook Heng Lee, Dedi Futra, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.

Original languageEnglish
Article number474
JournalNanoscale Research Letters
Volume12
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

cholera
Nanospheres
Polystyrenes
acrylic acid
bioinstrumentation
Biosensors
Acrylics
polystyrene
DNA
deoxyribonucleic acid
composite materials
Acids
Composite materials
Latex
latex
Latexes
Gold
gold
Nanoparticles
nanoparticles

Keywords

  • Biosensors
  • EDC/NHS chemistry
  • Electrochemical determination
  • Latex-gold nanosphere
  • Polystyrene co-acrylic acid (PSA) particles
  • Vibrio cholerae

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

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title = "Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres",
abstract = "An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5{\%}, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109{\%} (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5{\%} (n = 5). The electrochemical biosensor response was stable and maintainable at 95{\%} of its original response up to 58 days of storage period.",
keywords = "Biosensors, EDC/NHS chemistry, Electrochemical determination, Latex-gold nanosphere, Polystyrene co-acrylic acid (PSA) particles, Vibrio cholerae",
author = "Mahbubur Rahman and Lee, {Yook Heng} and Dedi Futra and {Tan @ Chong}, {Ling Ling}",
year = "2017",
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T1 - Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

AU - Rahman, Mahbubur

AU - Lee, Yook Heng

AU - Futra, Dedi

AU - Tan @ Chong, Ling Ling

PY - 2017

Y1 - 2017

N2 - An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.

AB - An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.

KW - Biosensors

KW - EDC/NHS chemistry

KW - Electrochemical determination

KW - Latex-gold nanosphere

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