Ultramicrotomy and structural analyses of two thermophilic non-sulfur photosynthetic bacteria

O. Faith Anthony Akinnuoye, O. Othman, Nazlina Ibrahim, H. Ainon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two photosynthetic non-sulfur (PSNS) bacteria identified as Rhodomicrobium vannielii (UKM4-5A) and Rhodopseudomonas palustris (UKM2-5A) were isolated from hot spring with temperature of about 50°C. They were cultivated anaerobically at a starting incubation temperature of 47°C for seven to ten days with ability to thrive at higher temperature of up to 50°C. Unique peculiarity was observed in both bacteria with vegetative growth and polar budding in all observed young active cells. Negatively stained Rhodomicrobium vannielii cells revealed exostructural multiflagellation and emerging hyphae from both ends of poles. Internal inclusions of both organisms had shown sensitive ultrastructural features such as intracellular poly-β- hydroxybutyrate (PHB) granules particularly at the conjoint polar ends of both mother and progeny cells of Rhodomicrobium vannielii while bacteriochlorophyll particles were observed only in Rhodopseudomonas palustris. Ultrastructural details were consistency-dependent especially in methods adopted in pre ultrathin microtomy.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalMalaysian Journal of Microscopy
Volume6
Issue number1
Publication statusPublished - 2010

Fingerprint

Rhodomicrobium
Microtomy
Rhodopseudomonas
bacteria
Bacteria
Temperature
microtomy
cells
Hot springs
Hot Springs
Hydroxybutyrates
Bacteriochlorophylls
progeny
Hyphae
organisms
Poles
emerging
poles
Stem Cells
inclusions

Keywords

  • Exostructure
  • Lamella
  • Photosynthetic
  • Thermophilic
  • Ultramicrotomy

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Instrumentation

Cite this

Ultramicrotomy and structural analyses of two thermophilic non-sulfur photosynthetic bacteria. / Akinnuoye, O. Faith Anthony; Othman, O.; Ibrahim, Nazlina; Ainon, H.

In: Malaysian Journal of Microscopy, Vol. 6, No. 1, 2010, p. 23-29.

Research output: Contribution to journalArticle

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