Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique

D. C. Chandrabalan, M. M. Clyde, Normah Mohd. Noor

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A study was conducted to develop an optimized methodology for cryopreservation of in vitro derived Fortunella polyandra shoot tips using vitrification-based techniques. Cryopreservation of F. polyandra shoot tips was successfully performed with the emphasis placed upon the aspects of preconditioning involving the pregrowth and preculture of explants. Preculture alone on Murashige and Skoog (MS) medium with 0.3 M sucrose for one day was not sufficient in giving survival after cryopreservation. A preliminary experiment involving pregrowth on MS medium with 0.72 M sucrose for three days produced a survival of 23.4% after cryopreservation. The usage of a combination of cryoprotectants in the preculture medium (MS with 0.3 M sucrose and 0.5 M ethylene glycol) proved to be beneficial in enhancing survival percentage of cryopreserved explants up to 40% when precultured for one day. However the highest percentage of shoot tip survival after cryopreservation (56.6%) was achieved when a two-step preconditioning method (pregrowth on MS medium with 0.72 M sucrose for three days followed by preculture on MS medium with 0.3 M sucrose for one day) was employed.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages289-296
Number of pages8
Volume908
Publication statusPublished - 30 Sep 2011

Publication series

NameActa Horticulturae
Volume908
ISSN (Print)05677572

Fingerprint

Fortunella
vitrification
cryopreservation
sucrose
explants
methodology
ethylene glycol
cryoprotectants
shoot tips

Keywords

  • Cryoprotectant
  • Ethylene glycol
  • Preculture
  • Pregrowth
  • Sucrose

ASJC Scopus subject areas

  • Horticulture

Cite this

Chandrabalan, D. C., Clyde, M. M., & Mohd. Noor, N. (2011). Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique. In Acta Horticulturae (Vol. 908, pp. 289-296). (Acta Horticulturae; Vol. 908).

Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique. / Chandrabalan, D. C.; Clyde, M. M.; Mohd. Noor, Normah.

Acta Horticulturae. Vol. 908 2011. p. 289-296 (Acta Horticulturae; Vol. 908).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chandrabalan, DC, Clyde, MM & Mohd. Noor, N 2011, Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique. in Acta Horticulturae. vol. 908, Acta Horticulturae, vol. 908, pp. 289-296.
Chandrabalan DC, Clyde MM, Mohd. Noor N. Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique. In Acta Horticulturae. Vol. 908. 2011. p. 289-296. (Acta Horticulturae).
Chandrabalan, D. C. ; Clyde, M. M. ; Mohd. Noor, Normah. / Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique. Acta Horticulturae. Vol. 908 2011. pp. 289-296 (Acta Horticulturae).
@inproceedings{1dce0859efcb4c008aa56b9f5167dc6b,
title = "Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique",
abstract = "A study was conducted to develop an optimized methodology for cryopreservation of in vitro derived Fortunella polyandra shoot tips using vitrification-based techniques. Cryopreservation of F. polyandra shoot tips was successfully performed with the emphasis placed upon the aspects of preconditioning involving the pregrowth and preculture of explants. Preculture alone on Murashige and Skoog (MS) medium with 0.3 M sucrose for one day was not sufficient in giving survival after cryopreservation. A preliminary experiment involving pregrowth on MS medium with 0.72 M sucrose for three days produced a survival of 23.4{\%} after cryopreservation. The usage of a combination of cryoprotectants in the preculture medium (MS with 0.3 M sucrose and 0.5 M ethylene glycol) proved to be beneficial in enhancing survival percentage of cryopreserved explants up to 40{\%} when precultured for one day. However the highest percentage of shoot tip survival after cryopreservation (56.6{\%}) was achieved when a two-step preconditioning method (pregrowth on MS medium with 0.72 M sucrose for three days followed by preculture on MS medium with 0.3 M sucrose for one day) was employed.",
keywords = "Cryoprotectant, Ethylene glycol, Preculture, Pregrowth, Sucrose",
author = "Chandrabalan, {D. C.} and Clyde, {M. M.} and {Mohd. Noor}, Normah",
year = "2011",
month = "9",
day = "30",
language = "English",
isbn = "9789066054707",
volume = "908",
series = "Acta Horticulturae",
pages = "289--296",
booktitle = "Acta Horticulturae",

}

TY - GEN

T1 - Two-step preconditioning - A feasible method for cryopreservation of fortunella polyandra shoot tips using vitrification technique

AU - Chandrabalan, D. C.

AU - Clyde, M. M.

AU - Mohd. Noor, Normah

PY - 2011/9/30

Y1 - 2011/9/30

N2 - A study was conducted to develop an optimized methodology for cryopreservation of in vitro derived Fortunella polyandra shoot tips using vitrification-based techniques. Cryopreservation of F. polyandra shoot tips was successfully performed with the emphasis placed upon the aspects of preconditioning involving the pregrowth and preculture of explants. Preculture alone on Murashige and Skoog (MS) medium with 0.3 M sucrose for one day was not sufficient in giving survival after cryopreservation. A preliminary experiment involving pregrowth on MS medium with 0.72 M sucrose for three days produced a survival of 23.4% after cryopreservation. The usage of a combination of cryoprotectants in the preculture medium (MS with 0.3 M sucrose and 0.5 M ethylene glycol) proved to be beneficial in enhancing survival percentage of cryopreserved explants up to 40% when precultured for one day. However the highest percentage of shoot tip survival after cryopreservation (56.6%) was achieved when a two-step preconditioning method (pregrowth on MS medium with 0.72 M sucrose for three days followed by preculture on MS medium with 0.3 M sucrose for one day) was employed.

AB - A study was conducted to develop an optimized methodology for cryopreservation of in vitro derived Fortunella polyandra shoot tips using vitrification-based techniques. Cryopreservation of F. polyandra shoot tips was successfully performed with the emphasis placed upon the aspects of preconditioning involving the pregrowth and preculture of explants. Preculture alone on Murashige and Skoog (MS) medium with 0.3 M sucrose for one day was not sufficient in giving survival after cryopreservation. A preliminary experiment involving pregrowth on MS medium with 0.72 M sucrose for three days produced a survival of 23.4% after cryopreservation. The usage of a combination of cryoprotectants in the preculture medium (MS with 0.3 M sucrose and 0.5 M ethylene glycol) proved to be beneficial in enhancing survival percentage of cryopreserved explants up to 40% when precultured for one day. However the highest percentage of shoot tip survival after cryopreservation (56.6%) was achieved when a two-step preconditioning method (pregrowth on MS medium with 0.72 M sucrose for three days followed by preculture on MS medium with 0.3 M sucrose for one day) was employed.

KW - Cryoprotectant

KW - Ethylene glycol

KW - Preculture

KW - Pregrowth

KW - Sucrose

UR - http://www.scopus.com/inward/record.url?scp=84855705112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855705112&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9789066054707

VL - 908

T3 - Acta Horticulturae

SP - 289

EP - 296

BT - Acta Horticulturae

ER -