Two dimensional protein map standardization of human bone marrow stromal cells

Min Hwei Ng, Saeid R. Doustjalali, Rajiv Pathak, Negar S. Sabet, Sok F. Khor, Lokanathan Yogeswaran, Ali N. Ghani, Aminuddin Saim, Marzalina Mansor, Ruszymah Idrus

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human bone marrow stromal cells (BMSCs) have been hailed as a promising source for cell therapy and tissue regeneration due to its multipotent nature. Bone marrow stromal cells differentiate into various tissues at the cues from its surrounding. Proteomics is a powerful tool to elucidate cellular mechanisms. The present study established a reproducible protocol to display the complete qualitative 2-D protein map of BMSCs. Results showed the standard optimization of the 2-D protein mapping at pH 3-10 with 60 μg protein loading in freshly isolated mononuclear cells, cultured BMSCs (P0) and BMSCs (P2). This protocol could be further used, along with identification of differentially expressed protein by mass spectrometry, for thorough understanding of the molecular events involved, which would enable a better control of the various differentiation processes of BMSCs.

Original languageEnglish
Pages (from-to)85-87
Number of pages3
JournalJournal of Proteomics and Bioinformatics
Volume6
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Mesenchymal Stromal Cells
Standardization
Bone
Proteins
Tissue regeneration
Cell- and Tissue-Based Therapy
Proteomics
Cues
Mass spectrometry
Regeneration
Cultured Cells
Mass Spectrometry
Tissue

Keywords

  • 2-D gel electrophoresis
  • Bone marrow stromal cell (BMSC)
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Computer Science Applications

Cite this

Two dimensional protein map standardization of human bone marrow stromal cells. / Ng, Min Hwei; Doustjalali, Saeid R.; Pathak, Rajiv; Sabet, Negar S.; Khor, Sok F.; Yogeswaran, Lokanathan; Ghani, Ali N.; Saim, Aminuddin; Mansor, Marzalina; Idrus, Ruszymah.

In: Journal of Proteomics and Bioinformatics, Vol. 6, No. 4, 2013, p. 85-87.

Research output: Contribution to journalArticle

Ng, Min Hwei ; Doustjalali, Saeid R. ; Pathak, Rajiv ; Sabet, Negar S. ; Khor, Sok F. ; Yogeswaran, Lokanathan ; Ghani, Ali N. ; Saim, Aminuddin ; Mansor, Marzalina ; Idrus, Ruszymah. / Two dimensional protein map standardization of human bone marrow stromal cells. In: Journal of Proteomics and Bioinformatics. 2013 ; Vol. 6, No. 4. pp. 85-87.
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