Two-dimensional order in mammalian pre-ocular tear film

P. G. Petrov, J. M. Thompson, Irman Abdul Rahman, R. E. Ellis, E. M. Green, F. Miano, C. P. Winlove

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We report a grazing incidence x-ray diffraction (GIXD) investigation of the surface lipid layer of the pre-ocular tear film. For the first time we demonstrate the existence of 2D order over a wide range of surface pressures in this system, with typical spicing of 3.75 Å and 4.16 Å independent of the monolayer surface pressure. Analogous lipid ordering is also found in an artificial lipid mixture of the major lipid components of the tear film, suggesting that the 2D ordering is set by generic lipid-lipid interactions. Fluorescence microscopy of the natural and artificial tear film mixture reveals the co-existence of a dilute and a much more condensed phase in the amphiphilic lipid matrix over the pressure range of 15-45 mN/m investigated by GIXD, plus an additional structure due to the much more hydrophobic part of the mixture. This evidence supports the previous hypothesis that tear film has a layered structure.

Original languageEnglish
Pages (from-to)1140-1146
Number of pages7
JournalExperimental Eye Research
Volume84
Issue number6
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Lipids
Tears
Pressure
X-Rays
Incidence
Fluorescence Microscopy

Keywords

  • fluorescence microscopy
  • Langmuir films
  • tear film lipid layer
  • x-ray diffraction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Petrov, P. G., Thompson, J. M., Abdul Rahman, I., Ellis, R. E., Green, E. M., Miano, F., & Winlove, C. P. (2007). Two-dimensional order in mammalian pre-ocular tear film. Experimental Eye Research, 84(6), 1140-1146. https://doi.org/10.1016/j.exer.2007.02.012

Two-dimensional order in mammalian pre-ocular tear film. / Petrov, P. G.; Thompson, J. M.; Abdul Rahman, Irman; Ellis, R. E.; Green, E. M.; Miano, F.; Winlove, C. P.

In: Experimental Eye Research, Vol. 84, No. 6, 06.2007, p. 1140-1146.

Research output: Contribution to journalArticle

Petrov, PG, Thompson, JM, Abdul Rahman, I, Ellis, RE, Green, EM, Miano, F & Winlove, CP 2007, 'Two-dimensional order in mammalian pre-ocular tear film', Experimental Eye Research, vol. 84, no. 6, pp. 1140-1146. https://doi.org/10.1016/j.exer.2007.02.012
Petrov, P. G. ; Thompson, J. M. ; Abdul Rahman, Irman ; Ellis, R. E. ; Green, E. M. ; Miano, F. ; Winlove, C. P. / Two-dimensional order in mammalian pre-ocular tear film. In: Experimental Eye Research. 2007 ; Vol. 84, No. 6. pp. 1140-1146.
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