Twins! Microsatellite analysis of two embryos within one egg case in oviparous elasmobranchs

Samantha A. Hook, Syafiq M. Musa, Daniel M. Ripley, Jean Denis Hibbitt, Bianka Grunow, Timo Moritz, Holly A. Shiels

Research output: Contribution to journalArticle

Abstract

Elasmobranchs display various reproductive modes, which have been key to their evolutionary success. In recent decades there has been a rise in the number of reported cases of foetal abnormalities including fertilised, double-embryos held within one egg capsule, hereafter referred to as twins. Previously, the occurrences of twin egg cases have been reported in two batoid and one shark species. We report the first cases of twins in three species of oviparous elasmobranchs: the undulate ray (Raja undulata), the nursehound (Scyliorhinus stellaris), and the small-spotted catshark (Scyliorhinus canicula). We investigated the genetic relationships between the twins in S. stellaris, and S. canicula using microsatellite markers. Whilst the S. stellaris twins displayed the same genotypes, we found that the S. canicula twin individuals arose through heteropaternal superfecundation. This is the first reported incidence of such a paternity in elasmobranchs. The relationship between environmental change and reproductive strategy in elasmobranchs is unclear and further research is needed to determine its effect on the prevalence and mechanisms of formation of elasmobranch twins.

Original languageEnglish
Article numbere0224397
JournalPLoS ONE
Volume14
Issue number12
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Oviparity
Elasmobranchii
egg masses
Microsatellite Repeats
Capsules
Ovum
embryo (animal)
Scyliorhinus canicula
Embryonic Structures
microsatellite repeats
Skates (Fish)
Sharks
Scyliorhinus
Raja
Genotype
paternity
sharks
genetic relationships
Incidence
Research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Hook, S. A., Musa, S. M., Ripley, D. M., Hibbitt, J. D., Grunow, B., Moritz, T., & Shiels, H. A. (2019). Twins! Microsatellite analysis of two embryos within one egg case in oviparous elasmobranchs. PLoS ONE, 14(12), [e0224397]. https://doi.org/10.1371/journal.pone.0224397

Twins! Microsatellite analysis of two embryos within one egg case in oviparous elasmobranchs. / Hook, Samantha A.; Musa, Syafiq M.; Ripley, Daniel M.; Hibbitt, Jean Denis; Grunow, Bianka; Moritz, Timo; Shiels, Holly A.

In: PLoS ONE, Vol. 14, No. 12, e0224397, 01.01.2019.

Research output: Contribution to journalArticle

Hook, SA, Musa, SM, Ripley, DM, Hibbitt, JD, Grunow, B, Moritz, T & Shiels, HA 2019, 'Twins! Microsatellite analysis of two embryos within one egg case in oviparous elasmobranchs', PLoS ONE, vol. 14, no. 12, e0224397. https://doi.org/10.1371/journal.pone.0224397
Hook, Samantha A. ; Musa, Syafiq M. ; Ripley, Daniel M. ; Hibbitt, Jean Denis ; Grunow, Bianka ; Moritz, Timo ; Shiels, Holly A. / Twins! Microsatellite analysis of two embryos within one egg case in oviparous elasmobranchs. In: PLoS ONE. 2019 ; Vol. 14, No. 12.
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