Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats

Norhaniza Aminudin, Abdullah Nur Atiqah Haizum, Hasni Misbah, Saiful A. Karsani, Ruby Husain, See Z. Hoe, Onn H. Hashim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Proteins that are associated with hypertension may be identified by comparing the 2-dimensional gel electrophoresis (2-DE) profiles of the sera of spontaneously hypertensive rats (SHR) with those generated from normotensive Spraque-Dawley rats (SDR).Results: Five proteins of high abundance were found to be significantly altered when the 2-DE serum profiles of the SHR were compared to those that were similarly generated from the SDR. Analysis by mass spectrometry and database search identified the proteins as retinol binding protein 4, complement C3, albumin (19.9 kDa fragment), alpha1 macroglobulin and alpha1 antiproteinase, which are all known to be associated with hypertension. The altered expression of the two latter proteins was found to be abrogated when similar analysis was performed on sera of the SHR that were treated with captopril.Conclusion: Our data suggests that serum alpha1 macroglobulin and alpha1 antiproteinase are potentially useful complementary biomolecular indicators for monitoring of hypertension.

Original languageEnglish
Article number17
JournalProteome Science
Volume10
Issue number1
DOIs
Publication statusPublished - 15 Mar 2012
Externally publishedYes

Fingerprint

Macroglobulins
Captopril
Inbred SHR Rats
Rats
Hypertension
Serum
Electrophoresis
Proteins
Gels
Retinol-Binding Proteins
Complement C3
Albumins
Mass Spectrometry
Databases
Mass spectrometry
Monitoring

Keywords

  • Biomarker
  • Hypertension
  • Proteomics
  • Serum proteins
  • Spontaneously hypertensive rats

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats. / Aminudin, Norhaniza; Nur Atiqah Haizum, Abdullah; Misbah, Hasni; Karsani, Saiful A.; Husain, Ruby; Hoe, See Z.; Hashim, Onn H.

In: Proteome Science, Vol. 10, No. 1, 17, 15.03.2012.

Research output: Contribution to journalArticle

Aminudin, Norhaniza ; Nur Atiqah Haizum, Abdullah ; Misbah, Hasni ; Karsani, Saiful A. ; Husain, Ruby ; Hoe, See Z. ; Hashim, Onn H. / Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats. In: Proteome Science. 2012 ; Vol. 10, No. 1.
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AU - Karsani, Saiful A.

AU - Husain, Ruby

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