Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties

Jordan Chia Yung Lai, How Yee Lai, Nalamolu Koteswara Rao, Shiow Fern Ng

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ethnopharmacological relevance Blechnum orientale Linn. (B. orientale) is a fern traditionally used by the natives as a poultice to treat wounds, boils, ulcers, blisters, abscesses, and sores on the skin. Aim of the study To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabetic ulcer wounds. Materials and methods The water extract from the leaves of B. orientale was separated from the crude methanolic extract and subjected to flash column chromatography techniques to produce concentrated fractions. These fractions were tested for phytochemical composition, tannin content, antioxidative and antibacterial activity. The bioactive fraction was formulated into a sodium carboxymethylcellulose hydrogel. The extract-loaded hydrogels were then characterized and tested on excision ulcer wounds of streptozotocin-induced diabetic rats. Wound size was measured for 14 days. Histopathological studies were conducted on the healed wound tissues to observe for epithelisation, fibroblast proliferation and angiogenesis. All possible mean values were subjected to statistical analysis using One-way ANOVA and post-hoc with Tukey's T-test (P

Original languageEnglish
Pages (from-to)277-289
Number of pages13
JournalJournal of Ethnopharmacology
Volume189
DOIs
Publication statusPublished - 2 Aug 2016

Fingerprint

Ferns
Hydrogel
Tannins
Ulcer
Wounds and Injuries
Therapeutics
Carboxymethylcellulose Sodium
Hydrogels
Phytochemicals
Blister
Streptozocin
Complex Mixtures
Population Groups
Wound Healing
Abscess
Chromatography
Analysis of Variance
Fibroblasts
Skin
Water

Keywords

  • Blechnum orientale linn.
  • Diabetes
  • Diabetic ulcers
  • Hydrogel
  • Topical formulation
  • Wound healing

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties. / Lai, Jordan Chia Yung; Lai, How Yee; Rao, Nalamolu Koteswara; Ng, Shiow Fern.

In: Journal of Ethnopharmacology, Vol. 189, 02.08.2016, p. 277-289.

Research output: Contribution to journalArticle

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