Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom

Intan Elya Suka, Bee Lynn Chew, Hoe Han Goh, Nurulhikma Md Isa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

PROTEOLYSIS 6 plays major role in the N-end rule pathway as N-recognin which functions as E3 ligase enzyme. It mediates ubiquitin processes that lead to degradation of unstable substrate protein. The aim of the current study is to transform the PRT6 gene into tomato (Solanum lycopersicum) from the cultivar Micro-Tom and to investigate its function in regulating ripening in tomato fruits. The PRT6-RNAi construct was successfully transformed into Agrobacterium C58 via heat shock method and transformed into seven days old cotyledon explants. Factors affecting transformation efficiency such as co-cultivation time and type of plant growth regulator combination were evaluated. Results from this study found that pre-cultured cotyledons from seven days old seedlings incubated for 2 days in co-cultivation medium increased shoot regeneration. Plant growth hormones zeatin combine with auxin produced a higher number of callus formation but lower shoot proliferation and transformation frequency compared to treatments of single plant hormone in the selection medium. Polymerase chain reaction (PCR) was performed on the regenerated shoots to confirm the integration of PRT6 fragment into the genome of transgenic plants. Based on PCR analysis, all putative shoots were positive transformants.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

tomatoes
polymerase chain reaction
plant growth regulators
auxins
pituitary hormones
hormones
fruits
genome
regeneration
genes
enzymes
shock
fragments
degradation
proteins
heat

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Suka, I. E., Chew, B. L., Goh, H. H., & Md Isa, N. (2018). Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020059] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027974

Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom. / Suka, Intan Elya; Chew, Bee Lynn; Goh, Hoe Han; Md Isa, Nurulhikma.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020059.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Suka, IE, Chew, BL, Goh, HH & Md Isa, N 2018, Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020059, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027974
Suka IE, Chew BL, Goh HH, Md Isa N. Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020059 https://doi.org/10.1063/1.5027974
Suka, Intan Elya ; Chew, Bee Lynn ; Goh, Hoe Han ; Md Isa, Nurulhikma. / Transformation of PRT6 RNAi construct into tomato (Solanum lycopersicum) cv. Micro-Tom. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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