Transformasi Gen Metalotionin (eiMT1) daripada Eleusine indica ke dalam Pokok Tembakau, Nicotiana tabacum Berperantaraan Agrobacterium tumefaciens

Translated title of the contribution: Transformation of metallothionein gene (eiMT1) from Eleusine indica into Tobacco, Nicotiana tabacum by Agrobacterium tumefaciens

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1 Citation (Scopus)

Abstract

Metallothioneins (MTs) are low molecular weight, cysteine-rich metal-binding proteins found in a wide range of organisms including bacteria, fungi, plant and animal. Plant MTs are thought to play a role in metal metabolism and detoxification via chelating of the metal ions. This unique feature results in the interest to isolate MT gene from goosegrass, Eleusine indica. eiMT1 cDNA was cloned into pBI121 binary vector for the transformation into tobacco by Agrobacterium tumefaciens. Screening of transgenic plants was conducted using three specially designed primers, CMTF and CMTR and 35SF and PMTR, and a pair of gene specific primers, MTFS2 and MTRS2. The primer pairs used successfully produced expected DNA fragments of 270 bp, 1.1 kb and 170 bp, respectively. Sequencing and sequence analysis of the 170 bp fragment was 100 % identical to the eiMT1. Gene expression study via reverse transcription- polymerase chain reaction approach showed that the transgene was successfully expressed in 11 of the 19 transgenic plants.

Original languageUndefined/Unknown
Pages (from-to)927-933
Number of pages7
JournalSains Malaysiana
Volume39
Issue number6
Publication statusPublished - Dec 2010

Fingerprint

Eleusine indica
metallothionein
Agrobacterium radiobacter
Nicotiana tabacum
transgenic plants
tobacco
metals
metal ions
transgenes
cysteine
binding proteins
genes
sequence analysis
reverse transcriptase polymerase chain reaction
molecular weight
screening
gene expression
fungi
metabolism
bacteria

Keywords

  • EiMT1
  • Eleusine indica
  • Metallothionein
  • Tobacco
  • Transformation

ASJC Scopus subject areas

  • General

Cite this

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title = "Transformasi Gen Metalotionin (eiMT1) daripada Eleusine indica ke dalam Pokok Tembakau, Nicotiana tabacum Berperantaraan Agrobacterium tumefaciens",
abstract = "Metallothioneins (MTs) are low molecular weight, cysteine-rich metal-binding proteins found in a wide range of organisms including bacteria, fungi, plant and animal. Plant MTs are thought to play a role in metal metabolism and detoxification via chelating of the metal ions. This unique feature results in the interest to isolate MT gene from goosegrass, Eleusine indica. eiMT1 cDNA was cloned into pBI121 binary vector for the transformation into tobacco by Agrobacterium tumefaciens. Screening of transgenic plants was conducted using three specially designed primers, CMTF and CMTR and 35SF and PMTR, and a pair of gene specific primers, MTFS2 and MTRS2. The primer pairs used successfully produced expected DNA fragments of 270 bp, 1.1 kb and 170 bp, respectively. Sequencing and sequence analysis of the 170 bp fragment was 100 {\%} identical to the eiMT1. Gene expression study via reverse transcription- polymerase chain reaction approach showed that the transgene was successfully expressed in 11 of the 19 transgenic plants.",
keywords = "EiMT1, Eleusine indica, Metallothionein, Tobacco, Transformation",
author = "Sidik, {Nik Marzuki} and Yazid, {Roslina Mat} and {Che Mohd. Zain}, {Che Radziah} and Ismanizan Ismail",
year = "2010",
month = "12",
language = "Undefined/Unknown",
volume = "39",
pages = "927--933",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "6",

}

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T1 - Transformasi Gen Metalotionin (eiMT1) daripada Eleusine indica ke dalam Pokok Tembakau, Nicotiana tabacum Berperantaraan Agrobacterium tumefaciens

AU - Sidik, Nik Marzuki

AU - Yazid, Roslina Mat

AU - Che Mohd. Zain, Che Radziah

AU - Ismail, Ismanizan

PY - 2010/12

Y1 - 2010/12

N2 - Metallothioneins (MTs) are low molecular weight, cysteine-rich metal-binding proteins found in a wide range of organisms including bacteria, fungi, plant and animal. Plant MTs are thought to play a role in metal metabolism and detoxification via chelating of the metal ions. This unique feature results in the interest to isolate MT gene from goosegrass, Eleusine indica. eiMT1 cDNA was cloned into pBI121 binary vector for the transformation into tobacco by Agrobacterium tumefaciens. Screening of transgenic plants was conducted using three specially designed primers, CMTF and CMTR and 35SF and PMTR, and a pair of gene specific primers, MTFS2 and MTRS2. The primer pairs used successfully produced expected DNA fragments of 270 bp, 1.1 kb and 170 bp, respectively. Sequencing and sequence analysis of the 170 bp fragment was 100 % identical to the eiMT1. Gene expression study via reverse transcription- polymerase chain reaction approach showed that the transgene was successfully expressed in 11 of the 19 transgenic plants.

AB - Metallothioneins (MTs) are low molecular weight, cysteine-rich metal-binding proteins found in a wide range of organisms including bacteria, fungi, plant and animal. Plant MTs are thought to play a role in metal metabolism and detoxification via chelating of the metal ions. This unique feature results in the interest to isolate MT gene from goosegrass, Eleusine indica. eiMT1 cDNA was cloned into pBI121 binary vector for the transformation into tobacco by Agrobacterium tumefaciens. Screening of transgenic plants was conducted using three specially designed primers, CMTF and CMTR and 35SF and PMTR, and a pair of gene specific primers, MTFS2 and MTRS2. The primer pairs used successfully produced expected DNA fragments of 270 bp, 1.1 kb and 170 bp, respectively. Sequencing and sequence analysis of the 170 bp fragment was 100 % identical to the eiMT1. Gene expression study via reverse transcription- polymerase chain reaction approach showed that the transgene was successfully expressed in 11 of the 19 transgenic plants.

KW - EiMT1

KW - Eleusine indica

KW - Metallothionein

KW - Tobacco

KW - Transformation

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