Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.)

Chun Hock Sim, Mansor Clyde Mahani, Chee Yen Choong, Idris Salma

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Introduction. Pulasan and lychee arc from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on denaturing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interruptions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalFruits
Volume60
Issue number6
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Nephelium
Litchi
Litchi chinensis
Microsatellite Repeats
microsatellite repeats
Polymerase Chain Reaction
loci
polyacrylamide
gels
genetic polymorphism

Keywords

  • Genetic markers
  • Genetic polymorphism
  • Litchi chinensis
  • Malaysia
  • Molecular biology
  • Nephelium ramboutan-ake
  • PCR

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.). / Sim, Chun Hock; Mahani, Mansor Clyde; Choong, Chee Yen; Salma, Idris.

In: Fruits, Vol. 60, No. 6, 11.2005, p. 379-385.

Research output: Contribution to journalArticle

Sim, Chun Hock ; Mahani, Mansor Clyde ; Choong, Chee Yen ; Salma, Idris. / Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.). In: Fruits. 2005 ; Vol. 60, No. 6. pp. 379-385.
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N2 - Introduction. Pulasan and lychee arc from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on denaturing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interruptions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan.

AB - Introduction. Pulasan and lychee arc from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on denaturing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interruptions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan.

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