Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties

Fei Ling Yap, Soon Keng Cheong, Radhakrishnan Ammu, Leong Chooi Fun

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immnophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalMalaysian Journal of Pathology
Volume31
Issue number2
Publication statusPublished - Dec 2009

Fingerprint

Differentiation Antigens
Mesenchymal Stromal Cells
Osteocytes
Adipogenesis
Interleukin-12
Adipocytes
Osteogenesis
Flow Cytometry
Plasmids
Cytokines
Genes

Keywords

  • Differentiation
  • Immunophenotyping
  • Mesenchymal stem cells
  • Transfection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties. / Yap, Fei Ling; Cheong, Soon Keng; Ammu, Radhakrishnan; Chooi Fun, Leong.

In: Malaysian Journal of Pathology, Vol. 31, No. 2, 12.2009, p. 113-120.

Research output: Contribution to journalArticle

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