Transcriptomic profiling of substrate-dependent autoaggregation in Pseudomonas putida CP1

Wan Syaidatul Aqma Wan Mohd Noor, Padraig Doolan, Brid Quilty

Research output: Contribution to journalArticle

Abstract

Aims: Pseudomonas putida CP1 is an interesting environmental isolate which exhibits substrate-dependent autoaggregation when the organism was grown on 0.5% (w/v) fructose. Autoaggregation is a process of a singlebacterial species to develop clumps of cells during a substrate stress. This study was carried out to investigate the genetic changes in the bacterium during aggregate formation. Methodology and results: P. putida CP1 was grown on 0.5% (w/v) fructose in batch culture at 30 °C and 150 rpm. The removal of fructose from the medium corresponded with aggregation of the cells which started after 8 h incubation. Microarray gene expression profiling using a P. putida KT2440 Genome Oligonucleotide Array (Progenika, Spain) showed that 838 genes involved in metabolism and adaptation were differentially expressed in P. putida CP1. Global transcriptomic profiling studies showed that P. putida CP1 growing on fructose resulted in the induction of genes encoding for proteins mainly involved in protein translation, ABC transporters, oxidative phosphorylation and twocomponent systems (TCS). Novel genes, associated with autoaggregation, were identified using transcriptomic analysis involved in ABC transporter, TCS, flagella assembly and lipopolysaccharide biosynthesis. It was also associated with the up-regulation of genes involved in the flagellar assembly including the fliE gene which encodes for the flagellar hookbasal body protein. Conclusion, significance and impact of study: The identification of new genes involved in autoaggregation formation is important to understand the molecular basis of strain variation and the mechanisms implicated in cell-cell communication.

Original languageEnglish
Pages (from-to)503-512
Number of pages10
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Pseudomonas putida
Fructose
ATP-Binding Cassette Transporters
Genes
Cell Aggregation
Batch Cell Culture Techniques
Flagella
Oxidative Phosphorylation
Protein Biosynthesis
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Cell Communication
Spain
Lipopolysaccharides
Proteins
Up-Regulation
Genome
Bacteria

Keywords

  • Autoaggregation
  • Pseudomonas putida CP1
  • Sugar metabolism

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Transcriptomic profiling of substrate-dependent autoaggregation in Pseudomonas putida CP1. / Wan Mohd Noor, Wan Syaidatul Aqma; Doolan, Padraig; Quilty, Brid.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 503-512.

Research output: Contribution to journalArticle

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