Transcriptomic Profiling of Rice Seedlings Inoculated with the Symbiotic Fungus Trichoderma asperellum SL2

Febri Doni, F. Fathurrahman, Muhamad Shakirin Mispan, Nurul Shamsinah Mohd Suhaimi, Wan Mohtar Wan Yusoff, Norman Uphoff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The fungal species Trichoderma is reported to have a significant impact on the growth and physiological performance of rice plants. However, the molecular mechanisms that induce these effects remain unspecified. Using next-generation sequencing technology, this study compared the differential expression of genes in rice seedlings that had been inoculated with Trichoderma asperellum SL2 with the gene expression in seedlings that had no such inoculation. The study showed that many genes related to plant growth enhancement and physiological functioning are differentially expressed in seedlings which have been symbiotically colonized by T. asperellum SL2. In these seedlings, specific genes related to photosynthesis, RNA activity, stomatal activity, and root development were found to be up-regulated as others were down-regulated. Although the exact causal mechanisms at the molecular level remain to be identified, the presence of Trichoderma versus its absence was associated with almost ten times more significant up-regulations than down-regulations for specific genes that have been identified from previous genomic mapping. Such analysis at the molecular level can help to explain observed phenotypic effects at the organismic level, and it begins to illuminate the observed beneficial relationships expressed phenotypically between crop plants and certain symbiotic microbes.

Original languageEnglish
JournalJournal of Plant Growth Regulation
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Trichoderma asperellum
transcriptomics
rice
fungi
seedlings
Trichoderma
microsymbionts
gene expression
genes
plant growth
photosynthesis
RNA
genomics
crops

Keywords

  • Gene expression
  • Rice
  • Transcriptomic analysis
  • Trichoderma

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Transcriptomic Profiling of Rice Seedlings Inoculated with the Symbiotic Fungus Trichoderma asperellum SL2. / Doni, Febri; Fathurrahman, F.; Mispan, Muhamad Shakirin; Suhaimi, Nurul Shamsinah Mohd; Wan Yusoff, Wan Mohtar; Uphoff, Norman.

In: Journal of Plant Growth Regulation, 01.01.2019.

Research output: Contribution to journalArticle

Doni, Febri ; Fathurrahman, F. ; Mispan, Muhamad Shakirin ; Suhaimi, Nurul Shamsinah Mohd ; Wan Yusoff, Wan Mohtar ; Uphoff, Norman. / Transcriptomic Profiling of Rice Seedlings Inoculated with the Symbiotic Fungus Trichoderma asperellum SL2. In: Journal of Plant Growth Regulation. 2019.
@article{b87f4d2d677449aea8232bce5a1b8676,
title = "Transcriptomic Profiling of Rice Seedlings Inoculated with the Symbiotic Fungus Trichoderma asperellum SL2",
abstract = "The fungal species Trichoderma is reported to have a significant impact on the growth and physiological performance of rice plants. However, the molecular mechanisms that induce these effects remain unspecified. Using next-generation sequencing technology, this study compared the differential expression of genes in rice seedlings that had been inoculated with Trichoderma asperellum SL2 with the gene expression in seedlings that had no such inoculation. The study showed that many genes related to plant growth enhancement and physiological functioning are differentially expressed in seedlings which have been symbiotically colonized by T. asperellum SL2. In these seedlings, specific genes related to photosynthesis, RNA activity, stomatal activity, and root development were found to be up-regulated as others were down-regulated. Although the exact causal mechanisms at the molecular level remain to be identified, the presence of Trichoderma versus its absence was associated with almost ten times more significant up-regulations than down-regulations for specific genes that have been identified from previous genomic mapping. Such analysis at the molecular level can help to explain observed phenotypic effects at the organismic level, and it begins to illuminate the observed beneficial relationships expressed phenotypically between crop plants and certain symbiotic microbes.",
keywords = "Gene expression, Rice, Transcriptomic analysis, Trichoderma",
author = "Febri Doni and F. Fathurrahman and Mispan, {Muhamad Shakirin} and Suhaimi, {Nurul Shamsinah Mohd} and {Wan Yusoff}, {Wan Mohtar} and Norman Uphoff",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s00344-019-09952-7",
language = "English",
journal = "Journal of Plant Growth Regulation",
issn = "0721-7595",
publisher = "Springer New York",

}

TY - JOUR

T1 - Transcriptomic Profiling of Rice Seedlings Inoculated with the Symbiotic Fungus Trichoderma asperellum SL2

AU - Doni, Febri

AU - Fathurrahman, F.

AU - Mispan, Muhamad Shakirin

AU - Suhaimi, Nurul Shamsinah Mohd

AU - Wan Yusoff, Wan Mohtar

AU - Uphoff, Norman

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The fungal species Trichoderma is reported to have a significant impact on the growth and physiological performance of rice plants. However, the molecular mechanisms that induce these effects remain unspecified. Using next-generation sequencing technology, this study compared the differential expression of genes in rice seedlings that had been inoculated with Trichoderma asperellum SL2 with the gene expression in seedlings that had no such inoculation. The study showed that many genes related to plant growth enhancement and physiological functioning are differentially expressed in seedlings which have been symbiotically colonized by T. asperellum SL2. In these seedlings, specific genes related to photosynthesis, RNA activity, stomatal activity, and root development were found to be up-regulated as others were down-regulated. Although the exact causal mechanisms at the molecular level remain to be identified, the presence of Trichoderma versus its absence was associated with almost ten times more significant up-regulations than down-regulations for specific genes that have been identified from previous genomic mapping. Such analysis at the molecular level can help to explain observed phenotypic effects at the organismic level, and it begins to illuminate the observed beneficial relationships expressed phenotypically between crop plants and certain symbiotic microbes.

AB - The fungal species Trichoderma is reported to have a significant impact on the growth and physiological performance of rice plants. However, the molecular mechanisms that induce these effects remain unspecified. Using next-generation sequencing technology, this study compared the differential expression of genes in rice seedlings that had been inoculated with Trichoderma asperellum SL2 with the gene expression in seedlings that had no such inoculation. The study showed that many genes related to plant growth enhancement and physiological functioning are differentially expressed in seedlings which have been symbiotically colonized by T. asperellum SL2. In these seedlings, specific genes related to photosynthesis, RNA activity, stomatal activity, and root development were found to be up-regulated as others were down-regulated. Although the exact causal mechanisms at the molecular level remain to be identified, the presence of Trichoderma versus its absence was associated with almost ten times more significant up-regulations than down-regulations for specific genes that have been identified from previous genomic mapping. Such analysis at the molecular level can help to explain observed phenotypic effects at the organismic level, and it begins to illuminate the observed beneficial relationships expressed phenotypically between crop plants and certain symbiotic microbes.

KW - Gene expression

KW - Rice

KW - Transcriptomic analysis

KW - Trichoderma

UR - http://www.scopus.com/inward/record.url?scp=85063203766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063203766&partnerID=8YFLogxK

U2 - 10.1007/s00344-019-09952-7

DO - 10.1007/s00344-019-09952-7

M3 - Article

JO - Journal of Plant Growth Regulation

JF - Journal of Plant Growth Regulation

SN - 0721-7595

ER -