Transcriptome-wide effects of expansin gene manipulation in etiolated Arabidopsis seedling

Iqmal Asyraf Ilias, Kohei Negishi, Keito Yasue, Naohiro Jomura, Kengo Morohashi, Syarul Nataqain Baharum, Hoe Han Goh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Expansin is a non-enzymatic protein which plays a pivotal role in cell wall loosening by inducing stress relaxation and extension in the plant cell wall. Previous studies on Arabidopsis, Petunia × hybrida, and tomato demonstrated that the suppression of expansin gene expression reduced plant growth but expansin overexpression does not necessarily promotes growth. In this study, both expansin gene suppression and overexpression in dark-grown transgenic Arabidopsis seedlings resulted in reduced hypocotyl length at late growth stages with a more pronounced effect for the overexpression. This defect in hypocotyl elongation raises questions about the molecular effect of expansin gene manipulation. RNA-seq analysis of the transcriptomic changes between day 3 and day 5 seedlings for both transgenic lines found numerous differentially expressed genes (DEGs) including transcription factors and hormone-related genes involved in different aspects of cell wall development. These DEGs imply that the observed hypocotyl growth retardation is a consequence of the concerted effect of regulatory factors and multiple cell-wall related genes, which are important for cell wall remodelling during rapid hypocotyl elongation. This is further supported by co-expression analysis through network-centric approach of differential network cluster analysis. This first transcriptome-wide study of expansin manipulation explains why the effect of expansin overexpression is greater than suppression and provides insights into the dynamic nature of molecular regulation during etiolation.

Original languageEnglish
JournalJournal of Plant Research
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

expansins
transcriptome
Arabidopsis
seedlings
hypocotyls
cell walls
genes
genetically modified organisms
etiolation
stress relaxation
Petunia hybrida
transcriptomics
growth retardation
cluster analysis
transcription factors
hormones
developmental stages
plant growth
tomatoes
RNA

Keywords

  • 3′ mRNA sequencing
  • Cell wall
  • Co-expression analysis
  • Etiolation
  • Expansin
  • Hypocotyl
  • QuantSeq
  • RNA-seq

ASJC Scopus subject areas

  • Plant Science

Cite this

Transcriptome-wide effects of expansin gene manipulation in etiolated Arabidopsis seedling. / Ilias, Iqmal Asyraf; Negishi, Kohei; Yasue, Keito; Jomura, Naohiro; Morohashi, Kengo; Baharum, Syarul Nataqain; Goh, Hoe Han.

In: Journal of Plant Research, 01.01.2018.

Research output: Contribution to journalArticle

Ilias, Iqmal Asyraf ; Negishi, Kohei ; Yasue, Keito ; Jomura, Naohiro ; Morohashi, Kengo ; Baharum, Syarul Nataqain ; Goh, Hoe Han. / Transcriptome-wide effects of expansin gene manipulation in etiolated Arabidopsis seedling. In: Journal of Plant Research. 2018.
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