Transcriptome-wide effect of DE-ETIOLATED1 (DET1) suppression in embryogenic callus of Carica papaya

Nur Diyana Jamaluddin, Emelda Rosseleena Rohani, Normah Mohd. Noor, Hoe Han Goh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Papaya is one of the most nutritional fruits, rich in vitamins, carotenoids, flavonoids and other antioxidants. Previous studies showed phytonutrient improvement without affecting quality in tomato fruit and rapeseed through the suppression of DE-ETIOLATED-1 (DET1), a negative regulator in photomorphogenesis. This study is conducted to study the effects of DET1 gene suppression in papaya embryogenic callus. Immature zygotic embryos were transformed with constitutive expression of a hairpin DET1 construct (hpDET1). PCR screening of transformed calli and reverse transcription quantitative PCR (RT-qPCR) verified that DET1 gene downregulation in two of the positive transformants. High-throughput cDNA 3′ ends sequencing on DET1-suppressed and control calli for transcriptomic analysis of global gene expression identified a total of 452 significant (FDR < 0.05) differentially expressed genes (DEGs) upon DET1 suppression. The 123 upregulated DEGs were mainly involved in phenylpropanoid biosynthesis and stress responses, compared to 329 downregulated DEGs involved in developmental processes, lipid metabolism, and response to various stimuli. This is the first study to demonstrate transcriptome-wide relationship between light-regulated pathway and secondary metabolite biosynthetic pathways in papaya. This further supports that the manipulation of regulatory gene involved in light-regulated pathway is possible for phytonutrient improvement of tropical fruit crops.

Original languageEnglish
JournalJournal of Plant Research
DOIs
Publication statusAccepted/In press - 1 Jan 2019

Fingerprint

Carica papaya
transcriptome
callus
papayas
genes
tropical and subtropical crops
photomorphogenesis
fruits
reverse transcription
tropical and subtropical fruits
fruit crops
phenylpropanoids
rapeseed
regulator genes
transcriptomics
embryo (plant)
lipid metabolism
secondary metabolites
biochemical pathways
vitamins

Keywords

  • DE-ETIOLATED1
  • Embryogenic callus
  • HpRNAi
  • Photomorphogenesis
  • RNA-seq

ASJC Scopus subject areas

  • Plant Science

Cite this

Transcriptome-wide effect of DE-ETIOLATED1 (DET1) suppression in embryogenic callus of Carica papaya. / Jamaluddin, Nur Diyana; Rohani, Emelda Rosseleena; Mohd. Noor, Normah; Goh, Hoe Han.

In: Journal of Plant Research, 01.01.2019.

Research output: Contribution to journalArticle

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