Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) transcript profiling was applied to generate the expression profiles of Polygonum minus in response to salicylic acid (SA) and methyl jasmonate (MeJA) elicitations. This study reveals two different sets of genes induced by SA and MeJA, respectively where stress-related genes were proved to lead to the expression of genes involved in plant secondary metabolite biosynthetic pathways. A total of 98 transcript-derived fragments (TDFs) were up-regulated, including 46 from SA-treated and 52 from MeJA-treated samples. The cDNA-AFLP transcripts generated using 64 different Mse1/Taq1 primer combinations showed that treatments with SA and MeJA induced genes mostly involved in scavenging reactive oxygen species, including zeaxanthin epoxidase, cytosolic ascorbate peroxidase 1 and peroxidase. Of these stress-related genes, 15 % of other annotated TDFs are involved mainly in secondary metabolic processes where among these, two genes encoding (+)-delta cadinene synthase and cinnamoyl-CoA reductase were highlighted.

Original languageEnglish
Pages (from-to)2231-2241
Number of pages11
JournalMolecular Biology Reports
Volume40
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Polygonum
Salicylic Acid
Gene Expression Profiling
Genes
cinnamoyl CoA reductase
Complementary DNA
Ascorbate Peroxidases
Secondary Metabolism
Biosynthetic Pathways
Peroxidase
Reactive Oxygen Species
methyl jasmonate
Gene Expression
Research

Keywords

  • cDNA-amplified fragment length polymorphism
  • Methyl jasmonate
  • Polygonum minus
  • Salicylic acid
  • Secondary metabolite
  • Stress-related gene

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus. / Ee, Su Fang; Oh, Ji Min; Mohd. Noor, Normah; Kwon, Taek Ryoun; Mohamed Hussein, Zeti Azura; Ismail, Ismanizan; Zainal, Zamri.

In: Molecular Biology Reports, Vol. 40, No. 3, 03.2013, p. 2231-2241.

Research output: Contribution to journalArticle

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