Transcriptome landscape of Rafflesia cantleyi floral buds reveals insights into the roles of transcription factors and phytohormones in flower development

Safoora Amini, Khadijah Rosli, Mohd Faizal Abu-Bakar, Halimah Alias, Mohd Noor Mat-Isa, Mohd Afiq Aizat Juhari, Jumaat Haji-Adam, Hoe Han Goh, Kiew Lian Wan

Research output: Contribution to journalArticle

Abstract

Rafflesia possesses unique biological features and known primarily for producing the world's largest and existing as a single flower. However, to date, little is known about key regulators participating in Rafflesia flower development. In order to further understand the molecular mechanism that regulates Rafflesia cantleyi flower development, RNA-seq data from three developmental stages of floral bud, representing the floral organ primordia initiation, floral organ differentiation, and floral bud outgrowth, were analysed. A total of 89,890 transcripts were assembled of which up to 35% could be annotated based on homology search. Advanced transcriptome analysis using K-mean clustering on the differentially expressed genes (DEGs) was able to identify 12 expression clusters that reflect major trends and key transitional states, which correlate to specific developmental stages. Through this, comparative gene expression analysis of different floral bud stages identified various transcription factors related to flower development. The members of WRKY, NAC, bHLH, and MYB families are the most represented among the DEGs, suggesting their important function in flower development. Furthermore, pathway enrichment analysis also revealed DEGs that are involved in various phytohormone signal transduction events such as auxin and auxin transport, cytokinin and gibberellin biosynthesis. Results of this study imply that transcription factors and phytohormone signalling pathways play major role in Rafflesia floral bud development. This study provides an invaluable resource for molecular studies of the flower development process in Rafflesia and other plant species.

Original languageEnglish
Article numbere0226338
JournalPLoS ONE
Volume14
Issue number12
DOIs
Publication statusPublished - 1 Jan 2019

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Rafflesia
Plant Growth Regulators
Transcriptome
transcriptome
plant hormones
Indoleacetic Acids
Transcription Factors
transcription factors
Genes
buds
flowering
Gibberellins
Cytokinins
Signal transduction
Biosynthesis
auxins
Gene expression
developmental stages
RNA
genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Transcriptome landscape of Rafflesia cantleyi floral buds reveals insights into the roles of transcription factors and phytohormones in flower development. / Amini, Safoora; Rosli, Khadijah; Abu-Bakar, Mohd Faizal; Alias, Halimah; Mat-Isa, Mohd Noor; Juhari, Mohd Afiq Aizat; Haji-Adam, Jumaat; Goh, Hoe Han; Wan, Kiew Lian.

In: PLoS ONE, Vol. 14, No. 12, e0226338, 01.01.2019.

Research output: Contribution to journalArticle

Amini, Safoora ; Rosli, Khadijah ; Abu-Bakar, Mohd Faizal ; Alias, Halimah ; Mat-Isa, Mohd Noor ; Juhari, Mohd Afiq Aizat ; Haji-Adam, Jumaat ; Goh, Hoe Han ; Wan, Kiew Lian. / Transcriptome landscape of Rafflesia cantleyi floral buds reveals insights into the roles of transcription factors and phytohormones in flower development. In: PLoS ONE. 2019 ; Vol. 14, No. 12.
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