Transactivator protein: An alternative for delivery of recombinant proteins for safer reprogramming of induced Pluripotent Stem Cell

Fazlina Nordin, Raja Norazireen Raja Ahmad, Farzin Farzaneh

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

Induced pluripotent stem cells (iPSC) are somatic cells reprogrammed to pluripotency by forced expression of pluripotency factors. These cells are shown to have the same pluripotent potential as embryonic stem cells (ESC) and considered as an alternative to the much controversial usage of ESC which involved human embryos. However, the traditional method in reprogramming cells into iPSC using genome-integrating retro- or lenti- viruses remains an obstacle for its application in clinical setting. Although numerous studies have been conducted for a safer DNA-based reprogramming, reprogramming of iPSC by genetic modifications may raise the possibility of malignant transformation and has been a major limitation for its usage in clinical applications. Therefore, there is a need for an alternative method to reprogram the cells without the use of gene editing and a much safer way to deliver transcription factors to induce pluripotency on target cells. Using protein transduction approach, a number of studies have demonstrated the generation of human iPSCs from human fibroblasts and mouse embryonic fibroblasts by direct delivery of reprogramming proteins. In this review, the definition and mechanism of HIV-TAT protein (a type of protein transduction domain) in delivering recombinant proteins, including the potential of protein-based delivery to induce iPSC were further discussed.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalVirus Research
Volume235
DOIs
Publication statusPublished - 2 May 2017

Fingerprint

Induced Pluripotent Stem Cells
Trans-Activators
Recombinant Proteins
Embryonic Stem Cells
Proteins
Fibroblasts
Human Immunodeficiency Virus Proteins
Transcription Factors
Embryonic Structures
Genome
Viruses
DNA

Keywords

  • iPSC
  • Protein transduction
  • Reprogramming somatic cell
  • TAT protein

ASJC Scopus subject areas

  • Cancer Research
  • Infectious Diseases
  • Virology

Cite this

Transactivator protein : An alternative for delivery of recombinant proteins for safer reprogramming of induced Pluripotent Stem Cell. / Nordin, Fazlina; Ahmad, Raja Norazireen Raja; Farzaneh, Farzin.

In: Virus Research, Vol. 235, 02.05.2017, p. 106-114.

Research output: Contribution to journalReview article

@article{f603b6b5d5db4ff9bc85d5acc86504e5,
title = "Transactivator protein: An alternative for delivery of recombinant proteins for safer reprogramming of induced Pluripotent Stem Cell",
abstract = "Induced pluripotent stem cells (iPSC) are somatic cells reprogrammed to pluripotency by forced expression of pluripotency factors. These cells are shown to have the same pluripotent potential as embryonic stem cells (ESC) and considered as an alternative to the much controversial usage of ESC which involved human embryos. However, the traditional method in reprogramming cells into iPSC using genome-integrating retro- or lenti- viruses remains an obstacle for its application in clinical setting. Although numerous studies have been conducted for a safer DNA-based reprogramming, reprogramming of iPSC by genetic modifications may raise the possibility of malignant transformation and has been a major limitation for its usage in clinical applications. Therefore, there is a need for an alternative method to reprogram the cells without the use of gene editing and a much safer way to deliver transcription factors to induce pluripotency on target cells. Using protein transduction approach, a number of studies have demonstrated the generation of human iPSCs from human fibroblasts and mouse embryonic fibroblasts by direct delivery of reprogramming proteins. In this review, the definition and mechanism of HIV-TAT protein (a type of protein transduction domain) in delivering recombinant proteins, including the potential of protein-based delivery to induce iPSC were further discussed.",
keywords = "iPSC, Protein transduction, Reprogramming somatic cell, TAT protein",
author = "Fazlina Nordin and Ahmad, {Raja Norazireen Raja} and Farzin Farzaneh",
year = "2017",
month = "5",
day = "2",
doi = "10.1016/j.virusres.2017.04.007",
language = "English",
volume = "235",
pages = "106--114",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",

}

TY - JOUR

T1 - Transactivator protein

T2 - An alternative for delivery of recombinant proteins for safer reprogramming of induced Pluripotent Stem Cell

AU - Nordin, Fazlina

AU - Ahmad, Raja Norazireen Raja

AU - Farzaneh, Farzin

PY - 2017/5/2

Y1 - 2017/5/2

N2 - Induced pluripotent stem cells (iPSC) are somatic cells reprogrammed to pluripotency by forced expression of pluripotency factors. These cells are shown to have the same pluripotent potential as embryonic stem cells (ESC) and considered as an alternative to the much controversial usage of ESC which involved human embryos. However, the traditional method in reprogramming cells into iPSC using genome-integrating retro- or lenti- viruses remains an obstacle for its application in clinical setting. Although numerous studies have been conducted for a safer DNA-based reprogramming, reprogramming of iPSC by genetic modifications may raise the possibility of malignant transformation and has been a major limitation for its usage in clinical applications. Therefore, there is a need for an alternative method to reprogram the cells without the use of gene editing and a much safer way to deliver transcription factors to induce pluripotency on target cells. Using protein transduction approach, a number of studies have demonstrated the generation of human iPSCs from human fibroblasts and mouse embryonic fibroblasts by direct delivery of reprogramming proteins. In this review, the definition and mechanism of HIV-TAT protein (a type of protein transduction domain) in delivering recombinant proteins, including the potential of protein-based delivery to induce iPSC were further discussed.

AB - Induced pluripotent stem cells (iPSC) are somatic cells reprogrammed to pluripotency by forced expression of pluripotency factors. These cells are shown to have the same pluripotent potential as embryonic stem cells (ESC) and considered as an alternative to the much controversial usage of ESC which involved human embryos. However, the traditional method in reprogramming cells into iPSC using genome-integrating retro- or lenti- viruses remains an obstacle for its application in clinical setting. Although numerous studies have been conducted for a safer DNA-based reprogramming, reprogramming of iPSC by genetic modifications may raise the possibility of malignant transformation and has been a major limitation for its usage in clinical applications. Therefore, there is a need for an alternative method to reprogram the cells without the use of gene editing and a much safer way to deliver transcription factors to induce pluripotency on target cells. Using protein transduction approach, a number of studies have demonstrated the generation of human iPSCs from human fibroblasts and mouse embryonic fibroblasts by direct delivery of reprogramming proteins. In this review, the definition and mechanism of HIV-TAT protein (a type of protein transduction domain) in delivering recombinant proteins, including the potential of protein-based delivery to induce iPSC were further discussed.

KW - iPSC

KW - Protein transduction

KW - Reprogramming somatic cell

KW - TAT protein

UR - http://www.scopus.com/inward/record.url?scp=85018778801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018778801&partnerID=8YFLogxK

U2 - 10.1016/j.virusres.2017.04.007

DO - 10.1016/j.virusres.2017.04.007

M3 - Review article

C2 - 28408207

AN - SCOPUS:85018778801

VL - 235

SP - 106

EP - 114

JO - Virus Research

JF - Virus Research

SN - 0168-1702

ER -